Detailed information of TA system TA135666

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 792573..793328
• Replicon: chromosome
• Accession: NZ_CP042478
• Organism: Citrobacter freundii strain C50

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7L6ILI8
• Locus tag: FR753_RS03970
• Protein ID: WP_047359063.1
• Coordinates: 792573..792950 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FR753_RS03975
• Protein ID: WP_162906152.1
• Coordinates: 793002..793328 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR753_RS03950	788595..790454	+	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-
FR753_RS03955	790926..791774	-	849	WP_088732394.1	DUF4942 domain-containing protein	-
FR753_RS03960	791853..792056	-	204	WP_047359061.1	DUF957 domain-containing protein	-
FR753_RS03965	792085..792576	-	492	WP_047359062.1	hypothetical protein	-
FR753_RS03970	792573..792950	-	378	WP_047359063.1	TA system toxin CbtA family protein	Toxin
FR753_RS03975	793002..793328	-	327	WP_162906152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FR753_RS03980	793385..793606	-	222	WP_000691982.1	DUF987 domain-containing protein	-
FR753_RS03985	793627..794106	-	480	WP_047359065.1	DNA repair protein RadC	-
FR753_RS03990	794118..794579	-	462	WP_047359066.1	antirestriction protein	-
FR753_RS03995	794676..794915	-	240	WP_047359067.1	DUF905 domain-containing protein	-
FR753_RS04000	794993..795466	-	474	WP_052951289.1	hypothetical protein	-
FR753_RS04005	795488..796183	-	696	WP_047359068.1	hypothetical protein	-
FR753_RS04010	796366..797067	-	702	WP_047359069.1	WYL domain-containing protein	-
FR753_RS04015	797283..798104	-	822	WP_032751326.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14078.86 Da
• Isoelectric Point: 7.0606

>T135666 WP_047359063.1 NZ_CP042478:c792950-792573 [Citrobacter freundii]
VQTQPLSSTHETSPRPSPVDIWQHLLNHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
SADTQSPLIGSIDILRARKATGLMTRHGYRPVTDLITGKYKKEQQ

Nucleotide

• Length: 378 bp

>T135666 NZ_CP042478:c792950-792573 [Citrobacter freundii]
GTGCAAACCCAACCACTATCCTCCACGCATGAGACCTCACCACGTCCGTCCCCCGTGGATATCTGGCAGCACCTCCTGAA
CCATCTGCTGGATCGTCACTACGGTCTGACGCTAAACGACACGCCATTTGGCAACGATGGCGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAACTTTATCGTGGAAAAGTACGACCTGGTCCGTACTGACAGACGCGGCTTT
AGCGCAGACACACAGTCACCGCTTATCGGCAGTATTGATATCCTCCGAGCCCGGAAGGCGACCGGGCTGATGACCCGTCA
TGGCTACAGACCTGTGACAGATCTCATTACCGGCAAATACAAAAAGGAACAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11955.62 Da
• Isoelectric Point: 6.9554

>AT135666 WP_162906152.1 NZ_CP042478:c793328-793002 [Citrobacter freundii]
ITEPWWGLKRSITPCFGARLVQEGNRLHYLGDRASIAGTFSDADLRHLDQAFPVQLKQLELMLVSGELNPRHQHCVTLYS
KGLTCEADSLGSHGYVYIAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT135666 NZ_CP042478:c793328-793002 [Citrobacter freundii]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGGCGACCGCGCGAGTATCGCCGGCACGTTCAGCGACGCGGATTTACGCCATCTTGACCAGGCGTTTCCGG
TACAGCTCAAACAGCTTGAGCTGATGCTGGTTTCCGGTGAACTCAATCCCCGCCATCAGCACTGCGTCACATTATACTCA
AAAGGGCTGACCTGCGAGGCCGACAGCCTCGGCTCGCATGGATACGTGTACATCGCGATTTACCCGACACCTGCAGCAAC
CGCGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6ILI8