Detailed information of TA system TA135649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 699212..699687
• Replicon: chromosome
• Accession: NZ_CP042393
• Organism: Leuconostoc citreum strain CBA3623

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FGL76_RS03445
• Protein ID: WP_036058531.1
• Coordinates: 699424..699687 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7L8V129
• Locus tag: FGL76_RS03440
• Protein ID: WP_004903014.1
• Coordinates: 699212..699427 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL76_RS03410	694405..694884	-	480	WP_147002276.1	hypothetical protein	-
FGL76_RS03415	694895..697147	-	2253	WP_147002277.1	toprim domain-containing protein	-
FGL76_RS03420	697144..697404	-	261	WP_004903004.1	hypothetical protein	-
FGL76_RS03425	697862..698245	+	384	WP_004903008.1	hypothetical protein	-
FGL76_RS03430	698256..698624	+	369	WP_004903009.1	hypothetical protein	-
FGL76_RS03435	698631..699152	+	522	WP_004903011.1	hypothetical protein	-
FGL76_RS03440	699212..699427	+	216	WP_004903014.1	hypothetical protein	Antitoxin
FGL76_RS03445	699424..699687	+	264	WP_036058531.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGL76_RS03450	700102..700332	+	231	WP_004903019.1	hypothetical protein	-
FGL76_RS03455	700329..700775	+	447	WP_004903021.1	hypothetical protein	-
FGL76_RS03460	700759..701082	+	324	WP_004903022.1	hypothetical protein	-
FGL76_RS03465	701104..701322	+	219	WP_004903024.1	hypothetical protein	-
FGL76_RS03470	701787..702038	+	252	WP_004903031.1	hypothetical protein	-
FGL76_RS03475	702001..702312	+	312	WP_036069642.1	hypothetical protein	-
FGL76_RS03490	702999..703250	-	252	WP_004903043.1	hypothetical protein	-
FGL76_RS03495	703243..703611	-	369	WP_004903045.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-69)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10312.79 Da
• Isoelectric Point: 10.1260

>T135649 WP_036058531.1 NZ_CP042393:699424-699687 [Leuconostoc citreum]
IMYRVTFTKQALKQLKKLDKFTQSTIYQWLQKNLDGTDNPRAHGKGLTANHSGEWRYRVGDYRILANIKDDEIIIEVFTI
GHRRDIY

Nucleotide

• Length: 264 bp

>T135649 NZ_CP042393:699424-699687 [Leuconostoc citreum]
ATAATGTATCGTGTTACTTTCACTAAACAAGCGCTTAAACAATTAAAAAAGTTAGATAAATTCACGCAAAGCACCATTTA
TCAATGGCTTCAAAAAAATCTGGACGGCACGGATAACCCACGTGCGCATGGAAAAGGATTAACCGCAAATCATTCAGGAG
AATGGCGTTACCGGGTTGGCGATTATCGTATTTTAGCCAACATTAAAGATGATGAAATTATCATTGAGGTATTCACCATT
GGGCATCGTCGCGATATTTACTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8159.38 Da
• Isoelectric Point: 4.4550

>AT135649 WP_004903014.1 NZ_CP042393:699212-699427 [Leuconostoc citreum]
MTTMTLRINQEDSELIKKFAATHDLSLSDFARQAMLEKIEDDYDIVELKKAMMADDGTRYTMAEVKKELGL

Nucleotide

• Length: 216 bp

>AT135649 NZ_CP042393:699212-699427 [Leuconostoc citreum]
ATGACAACAATGACACTACGTATCAATCAAGAAGACTCAGAGCTCATCAAGAAGTTTGCAGCAACTCATGATTTAAGTTT
ATCTGACTTCGCGCGTCAAGCTATGTTAGAAAAAATTGAAGATGATTATGATATTGTCGAATTGAAAAAAGCAATGATGG
CTGATGATGGCACGCGATATACTATGGCTGAAGTCAAAAAGGAATTAGGACTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7L8V129