Detailed information of TA system TA135643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): dhiT-vapB/DUF433(antitoxin)
• Location: 2953639..2954132
• Replicon: chromosome
• Accession: NZ_CP042382
• Organism: Pistricoccus aurantiacus strain CBA4606

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: FGL86_RS13985
• Protein ID: WP_147185152.1
• Coordinates: 2953639..2953899 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FGL86_RS13990
• Protein ID: WP_147185153.1
• Coordinates: 2953908..2954132 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL86_RS13940	2948643..2949641	-	999	WP_147185144.1	UDP-N-acetylglucosamine 4,6-dehydratase (inverting)	-
FGL86_RS13945	2949721..2949924	+	204	WP_147185145.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FGL86_RS13950	2949941..2950249	+	309	WP_147185146.1	HigA family addiction module antidote protein	-
FGL86_RS13955	2950378..2950773	-	396	WP_147185147.1	hypothetical protein	-
FGL86_RS13960	2950833..2951177	-	345	WP_186764411.1	DUF2335 domain-containing protein	-
FGL86_RS17960	2951202..2951357	-	156	WP_186764412.1	hypothetical protein	-
FGL86_RS13965	2951347..2951556	-	210	WP_147185149.1	hypothetical protein	-
FGL86_RS13970	2951603..2952664	-	1062	WP_147185150.1	transposase	-
FGL86_RS13975	2952686..2953102	+	417	WP_147184266.1	IS200/IS605 family transposase	-
FGL86_RS13980	2953394..2953633	-	240	WP_147185151.1	DUF2442 domain-containing protein	-
FGL86_RS13985	2953639..2953899	-	261	WP_147185152.1	DUF4160 domain-containing protein	Toxin
FGL86_RS13990	2953908..2954132	-	225	WP_147185153.1	DUF433 domain-containing protein	Antitoxin
FGL86_RS13995	2954233..2956041	-	1809	WP_147185154.1	ABC transporter ATP-binding protein/permease	-
FGL86_RS14000	2956175..2957587	-	1413	WP_147185155.1	MBL fold metallo-hydrolase	-
FGL86_RS17965	2957599..2958864	-	1266	WP_186764524.1	exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2931340..2954132	22792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-70)

Antitoxin

• Protein of unknown function DUF433 (7-62)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10225.77 Da
• Isoelectric Point: 4.8278

>T135643 WP_147185152.1 NZ_CP042382:c2953899-2953639 [Pistricoccus aurantiacus]
MPTISAFFGILIRMYYDDHNPPHFHAYYGEYEAIITIQTLELMEGKLPRRALAMVVEWAVEHREQLMEDWALAEQHRPLN
KIEPLE

Nucleotide

• Length: 261 bp

>T135643 NZ_CP042382:c2953899-2953639 [Pistricoccus aurantiacus]
ATGCCAACGATCTCAGCGTTCTTCGGCATCTTGATTCGCATGTATTACGATGATCATAACCCGCCACACTTCCATGCTTA
CTACGGCGAATATGAAGCGATCATCACAATTCAAACTCTGGAACTCATGGAAGGTAAACTGCCGAGGCGTGCACTAGCGA
TGGTGGTGGAGTGGGCCGTTGAGCATCGTGAGCAGCTTATGGAAGATTGGGCTCTAGCAGAGCAACACAGGCCACTTAAC
AAGATCGAGCCTTTGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8374.52 Da
• Isoelectric Point: 4.3304

>AT135643 WP_147185153.1 NZ_CP042382:c2954132-2953908 [Pistricoccus aurantiacus]
MSELHRITYDPELCGGRPCIRGMRIRVKDILDLLAADATREEILEDFPYLETEDITAALEYAARQSDHPVLSVA

Nucleotide

• Length: 225 bp

>AT135643 NZ_CP042382:c2954132-2953908 [Pistricoccus aurantiacus]
ATGAGCGAATTGCACCGTATAACATACGATCCTGAATTATGTGGTGGACGCCCTTGTATTCGTGGCATGCGCATTCGTGT
CAAAGACATTTTAGATCTCTTGGCCGCTGATGCTACACGCGAAGAGATACTGGAAGATTTTCCTTATCTTGAAACCGAGG
ATATCACCGCTGCGCTAGAATACGCCGCTCGCCAAAGCGATCATCCTGTACTATCGGTCGCATAG