Detailed information of TA system TA135630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 13991..14540
• Replicon: plasmid pC54_003
• Accession: NZ_CP042367
• Organism: Acinetobacter pittii strain C54

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9KJ78
• Locus tag: FR838_RS20785
• Protein ID: WP_000176412.1
• Coordinates: 14250..14540 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9KCM7
• Locus tag: FR838_RS20780
• Protein ID: WP_005140357.1
• Coordinates: 13991..14266 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR838_RS20760	10326..11207	+	882	WP_005804946.1	replication initiation protein RepM	-
FR838_RS20765	11271..11837	+	567	WP_000858429.1	plasmid replication DNA-binding protein	-
FR838_RS20770	12188..12505	+	318	WP_005065053.1	hypothetical protein	-
FR838_RS20775	13035..13742	+	708	WP_038343693.1	toll/interleukin-1 receptor domain-containing protein	-
FR838_RS21030	13843..13986	+	144	WP_162899358.1	hypothetical protein	-
FR838_RS20780	13991..14266	+	276	WP_005140357.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FR838_RS20785	14250..14540	+	291	WP_000176412.1	type II toxin-antitoxin system YafQ family toxin	Toxin
FR838_RS21035	14588..14734	-	147	WP_000192351.1	hypothetical protein	-
FR838_RS20795	15214..16380	+	1167	Protein_21	IS3-like element ISAba22 family transposase	-
FR838_RS20800	17349..18656	-	1308	WP_086374879.1	NAD(P)/FAD-dependent oxidoreductase	-
FR838_RS20805	18673..19506	-	834	WP_086374880.1	S-formylglutathione hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25906	25906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (9-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11187.12 Da
• Isoelectric Point: 6.9783

>T135630 WP_000176412.1 NZ_CP042367:14250-14540 [Acinetobacter pittii]
MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTVPDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDN
NELILARIGSHSELFG

Nucleotide

• Length: 291 bp

>T135630 NZ_CP042367:14250-14540 [Acinetobacter pittii]
ATGACACTGACGATTAGGTACAAGAAACAATTTAAAAAAGATTTTAAACGGGTAAGCTCAAACCCAAAGCTTAAGAAAGG
ACTCTTAATTTTGAAGGATCTTATTGATGAGCATTTGGTAAAAACAGGGACTGTTCCAGATGAGTATTTACCTCATCCTT
TGATTGGTGAGTGGAAACCCCATATGGAATGCCATATTTTACCTGATTTATTATTAATTTGGGATGTGGATGAAGATAAT
AATGAGCTAATACTTGCTCGGATTGGATCTCATTCTGAACTTTTTGGATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10290.65 Da
• Isoelectric Point: 4.6702

>AT135630 WP_005140357.1 NZ_CP042367:13991-14266 [Acinetobacter pittii]
MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQVVNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQE
LFTDLEDDTDD

Nucleotide

• Length: 276 bp

>AT135630 NZ_CP042367:13991-14266 [Acinetobacter pittii]
ATGAATACTTTGGCAAAAAAAGATGAGTATGTACGTGCTAGGGTTCCAAATGAATTAAAGGTTCAAGCGGAGCGAATTCT
AAAAGAAGTGGGTTTAAATACTACAGATGCTATTCGCTTATTATTAACGCAAGTAGTAAATCGAGGTGGATTTCCTTTAG
AGTTAAGAACCCCTAACCGTACAACAATTGAGGCTATGAACTCTGTTGCAGAACCTAAAAAATTTAGTTCTGCTCAGGAA
TTGTTTACGGATTTAGAAGATGACACTGACGATTAG

Structures

Toxin

• AlphaFold DB: N9KJ78

Antitoxin

• AlphaFold DB: N9KCM7