Detailed information of TA system TA135625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 106377..106961
• Replicon: plasmid pC54_001
• Accession: NZ_CP042365
• Organism: Acinetobacter pittii strain C54

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: FR838_RS19540
• Protein ID: WP_000286964.1
• Coordinates: 106377..106679 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FR838_RS19545
• Protein ID: WP_000985609.1
• Coordinates: 106680..106961 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR838_RS19505	101503..102207	+	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
FR838_RS19510	102335..102673	-	339	WP_032073153.1	hypothetical protein	-
FR838_RS19515	102732..103502	+	771	WP_032073134.1	hypothetical protein	-
FR838_RS19520	103743..104030	+	288	WP_000438825.1	BrnT family toxin	-
FR838_RS19525	104017..104331	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FR838_RS19535	105839..106228	-	390	Protein_112	hypothetical protein	-
FR838_RS19540	106377..106679	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FR838_RS19545	106680..106961	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
FR838_RS19550	107041..107460	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FR838_RS19555	107583..108748	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
FR838_RS19560	108901..110235	+	1335	WP_033917544.1	ISNCY family transposase	-
FR838_RS19565	110366..110686	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FR838_RS19570	110679..110951	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / aph(3')-VIa / sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19 / blaOXA-58	-	1..256887	256887
	-	inside	IScluster/Tn	sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19	-	98009..133938	35929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T135625 WP_000286964.1 NZ_CP042365:106377-106679 [Acinetobacter pittii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T135625 NZ_CP042365:106377-106679 [Acinetobacter pittii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT135625 WP_000985609.1 NZ_CP042365:106680-106961 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT135625 NZ_CP042365:106680-106961 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94