135624

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 103743..104331 Replicon plasmid pC54_001
Accession NZ_CP042365
Organism Acinetobacter pittii strain C54

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag FR838_RS19520 Protein ID WP_000438825.1
Coordinates 103743..104030 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag FR838_RS19525 Protein ID WP_004728120.1
Coordinates 104017..104331 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR838_RS19480 98792..99139 - 348 Protein_102 IS4 family transposase -
FR838_RS19485 99229..100044 + 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
FR838_RS19490 100131..100373 + 243 Protein_104 phosphoglucosamine mutase -
FR838_RS19500 100609..101439 - 831 Protein_105 IS91-like element ISVsa3 family transposase -
FR838_RS19505 101503..102207 + 705 WP_001067784.1 IS6-like element IS1006 family transposase -
FR838_RS19510 102335..102673 - 339 WP_032073153.1 hypothetical protein -
FR838_RS19515 102732..103502 + 771 WP_032073134.1 hypothetical protein -
FR838_RS19520 103743..104030 + 288 WP_000438825.1 BrnT family toxin Toxin
FR838_RS19525 104017..104331 + 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
FR838_RS19535 105839..106228 - 390 Protein_112 hypothetical protein -
FR838_RS19540 106377..106679 + 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
FR838_RS19545 106680..106961 + 282 WP_000985609.1 putative addiction module antidote protein -
FR838_RS19550 107041..107460 - 420 WP_001180106.1 SMI1/KNR4 family protein -
FR838_RS19555 107583..108748 + 1166 WP_085940844.1 IS3-like element ISAba22 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / aph(3')-VIa / sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19 / blaOXA-58 - 1..256887 256887
- inside IScluster/Tn sul2 / msr(E) / mph(E) / floR / sul1 / qacE / catB3 / blaIMP-26 / dfrA19 - 98009..133938 35929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T135624 WP_000438825.1 NZ_CP042365:103743-104030 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T135624 NZ_CP042365:103743-104030 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT135624 WP_004728120.1 NZ_CP042365:104017-104331 [Acinetobacter pittii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT135624 NZ_CP042365:104017-104331 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References