Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1099038..1099660 Replicon chromosome
Accession NZ_CP042363
Organism Serratia plymuthica strain A294

Toxin (Protein)


Gene name Hha Uniprot ID S4YCZ4
Locus tag FS592_RS05100 Protein ID WP_006322256.1
Coordinates 1099038..1099241 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S4YKH5
Locus tag FS592_RS05105 Protein ID WP_004949348.1
Coordinates 1099292..1099660 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS592_RS05075 (FS592_05135) 1094805..1095266 + 462 WP_006323963.1 type II secretion system protein GspM -
FS592_RS05080 (FS592_05140) 1095299..1096129 + 831 WP_064798676.1 A24 family peptidase -
FS592_RS05085 (FS592_05145) 1096126..1096500 - 375 WP_006323969.1 type II secretion system pilot lipoprotein GspS -
FS592_RS05090 (FS592_05150) 1096780..1098201 + 1422 WP_020438550.1 N-acetylglucosamine-binding protein GbpA -
FS592_RS05095 (FS592_05155) 1098262..1098798 + 537 WP_006323971.1 cytochrome b/b6 domain-containing protein -
FS592_RS05100 (FS592_05160) 1099038..1099241 - 204 WP_006322256.1 HHA domain-containing protein Toxin
FS592_RS05105 (FS592_05165) 1099292..1099660 - 369 WP_004949348.1 Hha toxicity modulator TomB Antitoxin
FS592_RS05110 (FS592_05170) 1099820..1100173 - 354 WP_064798678.1 hypothetical protein -
FS592_RS05115 (FS592_05175) 1100625..1101338 + 714 WP_006323974.1 ABC transporter ATP-binding protein -
FS592_RS05120 (FS592_05180) 1101335..1102192 + 858 WP_004949351.1 metal ABC transporter permease -
FS592_RS05125 (FS592_05185) 1102218..1103096 + 879 WP_006323980.1 metal ABC transporter substrate-binding protein -
FS592_RS05130 (FS592_05190) 1103211..1103351 - 141 WP_004949353.1 type B 50S ribosomal protein L36 -
FS592_RS05135 (FS592_05195) 1103364..1103618 - 255 WP_004949355.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8085.45 Da        Isoelectric Point: 6.9764

>T135609 WP_006322256.1 NZ_CP042363:c1099241-1099038 [Serratia plymuthica]
MTKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Download         Length: 204 bp

>T135609 NZ_CP042363:c1099241-1099038 [Serratia plymuthica]
ATGACTAAAATTGACTATCTGATGCGTTTACGTAAATGCACCACGATTGACACCCTGGAACGCGTTATTGAAAAAAATAA
GTATGAGCTTTCGGATGACGAACTAGAGCTGTTCTACTCTGCAGCGGACCACCGTTTGGCCGAGCTCACCATGAATAAGC
TGTACGATAAAATCCCCGTTGCCGTTTGGAAATTCGTACGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14203.92 Da        Isoelectric Point: 4.5140

>AT135609 WP_004949348.1 NZ_CP042363:c1099660-1099292 [Serratia plymuthica]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEGICTTMKT

Download         Length: 369 bp

>AT135609 NZ_CP042363:c1099660-1099292 [Serratia plymuthica]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
AACGCTGGGCGACAGCCACCACGGCTGGGTGAACGATCCAACGTCTTCCGTCAACCTCCAGCTTAACGAGTTGATTGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAAAGTGATTTATCGGAGTTAGTCGAAGAGTATCTC
GACGATACCTACACGCTGTTTAGCAGTTATGGTATTAATGATTCCGATTTGCGCCGCTGGCAAAAAACCAAAGCGCGATT
ATTCAGAATGTTCTCAGGAGAGGGCATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1KM53

Antitoxin

Source ID Structure
AlphaFold DB S4YKH5

References