Detailed information of TA system TA135588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 105770..106506
• Replicon: plasmid unnamed2
• Accession: NZ_CP042359
• Organism: Rahnella aquatilis strain H145

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FS594_RS26550
• Protein ID: WP_235174919.1
• Coordinates: 106027..106506 (+)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H2J2E1
• Locus tag: FS594_RS26545
• Protein ID: WP_014333867.1
• Coordinates: 105770..106030 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS594_RS26515 (FS594_26695)	100966..101586	-	621	WP_217137531.1	LysE family translocator	-
FS594_RS26520 (FS594_26700)	101656..102381	-	726	WP_217137530.1	type 1 glutamine amidotransferase	-
FS594_RS26525 (FS594_26705)	102449..103741	-	1293	WP_235174911.1	transcriptional regulator PtsJ	-
FS594_RS26530 (FS594_26710)	103810..104616	+	807	WP_235174913.1	YggS family pyridoxal phosphate-dependent enzyme	-
FS594_RS26535	104613..104975	+	363	WP_235174915.1	hypothetical protein	-
FS594_RS26540	104956..105417	+	462	WP_235174917.1	PfkB family carbohydrate kinase	-
FS594_RS26545 (FS594_26720)	105770..106030	+	261	WP_014333867.1	DUF1778 domain-containing protein	Antitoxin
FS594_RS26550 (FS594_26725)	106027..106506	+	480	WP_235174919.1	GNAT family N-acetyltransferase	Toxin
FS594_RS26555 (FS594_26730)	106598..107647	-	1050	WP_235174921.1	tyrosine-type recombinase/integrase	-
FS594_RS26560 (FS594_26735)	108447..109565	-	1119	WP_217137526.1	enolase C-terminal domain-like protein	-
FS594_RS26565 (FS594_26740)	109558..110244	-	687	WP_217137525.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkH / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..467533	467533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17348.02 Da
• Isoelectric Point: 8.9404

>T135588 WP_235174919.1 NZ_CP042359:106027-106506 [Rahnella aquatilis]
MSLKLSAPEPLTTEHILDAFGCGESTLDEWLKRRALTNHLNGASRTFVVATHDKSIVGYYALAAGAVSHREATGSVRRNM
PDPVPVIVLGRLAVDLRTQGLKLGASLLQDAVLRVHSIAENTGVRALLVHALHEPAKQFYEHYGFTSSPINPMTLMLRL

Nucleotide

• Length: 480 bp

>T135588 NZ_CP042359:106027-106506 [Rahnella aquatilis]
ATGAGTCTAAAACTGTCTGCTCCTGAGCCACTTACAACTGAACATATCCTTGATGCGTTTGGTTGTGGGGAATCAACGTT
AGATGAATGGTTAAAACGACGCGCTCTGACAAACCATTTAAACGGTGCAAGTCGTACTTTTGTTGTCGCGACTCACGATA
AGAGTATTGTCGGTTATTATGCGCTCGCAGCCGGCGCTGTGTCGCACAGGGAAGCAACAGGGTCTGTTCGGCGTAATATG
CCTGATCCAGTTCCGGTCATAGTATTGGGAAGATTGGCGGTCGACCTTCGCACTCAGGGATTAAAGCTAGGTGCGTCATT
GCTACAGGATGCCGTTCTTCGAGTTCATTCAATTGCTGAAAACACAGGTGTCAGAGCATTGCTCGTACATGCACTTCATG
AACCGGCTAAACAATTTTATGAACACTACGGTTTTACATCATCGCCAATTAATCCGATGACATTAATGCTACGCCTTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9746.24 Da
• Isoelectric Point: 4.7527

>AT135588 WP_014333867.1 NZ_CP042359:105770-106030 [Rahnella aquatilis]
MRDAAINLRALPEQRDLIDQAASLLGKNRSDFMLEVACERAQAILLDQVFFQLDTEKFHEFTAMLDAPQKANQGLERLMA
VKAPWA

Nucleotide

• Length: 261 bp

>AT135588 NZ_CP042359:105770-106030 [Rahnella aquatilis]
ATGCGTGACGCTGCGATTAATTTGCGCGCTTTGCCGGAACAACGTGATCTCATCGACCAAGCCGCCAGCCTATTGGGTAA
AAACCGATCTGATTTTATGCTGGAAGTAGCGTGTGAACGTGCGCAGGCTATTCTGCTCGACCAGGTTTTCTTCCAGTTAG
ATACTGAAAAATTCCATGAATTCACAGCGATGCTTGACGCACCTCAAAAGGCAAATCAGGGTCTGGAACGGCTGATGGCA
GTGAAAGCACCTTGGGCATGA

Structures

Antitoxin

• AlphaFold DB: H2J2E1