Detailed information of TA system TA135582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3801941..3802558
• Replicon: chromosome
• Accession: NZ_CP042357
• Organism: Rahnella aquatilis strain H145

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: FS594_RS17930
• Protein ID: WP_013576617.1
• Coordinates: 3802355..3802558 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: FS594_RS17925
• Protein ID: WP_129952820.1
• Coordinates: 3801941..3802309 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS594_RS17895 (FS594_17960)	3797947..3798201	+	255	WP_120506405.1	type B 50S ribosomal protein L31	-
FS594_RS17900 (FS594_17965)	3798223..3798363	+	141	WP_013576611.1	type B 50S ribosomal protein L36	-
FS594_RS17905 (FS594_17970)	3798486..3799364	-	879	WP_129992124.1	metal ABC transporter substrate-binding protein	-
FS594_RS17910 (FS594_17975)	3799393..3800250	-	858	WP_217139370.1	metal ABC transporter permease	-
FS594_RS17915 (FS594_17980)	3800247..3800975	-	729	WP_205951586.1	ABC transporter ATP-binding protein	-
FS594_RS17920 (FS594_17985)	3801356..3801709	+	354	WP_129992079.1	hypothetical protein	-
FS594_RS17925 (FS594_17990)	3801941..3802309	+	369	WP_129952820.1	Hha toxicity modulator TomB	Antitoxin
FS594_RS17930 (FS594_17995)	3802355..3802558	+	204	WP_013576617.1	HHA domain-containing protein	Toxin
FS594_RS17935 (FS594_18000)	3802634..3803590	-	957	WP_235173217.1	LysR family transcriptional regulator	-
FS594_RS17940 (FS594_18005)	3803730..3805517	+	1788	WP_235173218.1	adenine deaminase C-terminal domain-containing protein	-
FS594_RS17945 (FS594_18010)	3805592..3806941	+	1350	WP_235173219.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T135582 WP_013576617.1 NZ_CP042357:3802355-3802558 [Rahnella aquatilis]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T135582 NZ_CP042357:3802355-3802558 [Rahnella aquatilis]
ATGAACAAAATCGACTATTTGATGCGTTTAAGAAAATGCACCACAATCGATACACTCGAACGTGTTATTGAGAAAAATAA
GTATGAACTTTCCGACGACGAACTGGAATTGTTTTACTCGGCAGCTGACCATCGTTTAGCGGAGCTAACGATGAACAAAC
TGTACGATAAAATTCCGGTATCAGTCTGGAAATTTGTACGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14347.14 Da
• Isoelectric Point: 4.8936

>AT135582 WP_129952820.1 NZ_CP042357:3801941-3802309 [Rahnella aquatilis]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPADNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT135582 NZ_CP042357:3801941-3802309 [Rahnella aquatilis]
ATGGATGAGTACTCGCCTAAGCGGCACGATATCGCTCAGTTACGCTACCTGAATGAAATGCTGTACGATGAAGGGATTGC
AACACTGGGTGACAGCCATCATGGCTGGGTTAATGATCCGACTTCGGCCGTTAACCTACAGCTCAATGAACTGATTGAGC
ACATTGCATCCTTTGTGATGAGTTTTAAAATTAAGTATCCGGCTGATAACCATTTAAGTGATCTGGTTGAAGAGTATCTG
GATGATACCTACACCCTGTTCAGCAATTATGGAATTAACGATTCTGATTTGCGTCAATGGCAAAAGACCAAGAAGCGCTT
ATTCAGAATGTTCTCAGGGGACTACATCTGTACCCTAATGAAGACTTGA

Structures

Toxin

• AlphaFold DB: H8NYI7