Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1077763..1078394 Replicon chromosome
Accession NZ_CP042353
Organism Serratia rubidaea strain H469

Toxin (Protein)


Gene name Hha Uniprot ID L0MF14
Locus tag FS596_RS05075 Protein ID WP_015671220.1
Coordinates 1077763..1077966 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A140F0B9
Locus tag FS596_RS05080 Protein ID WP_061324804.1
Coordinates 1078026..1078394 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS596_RS05055 (FS596_05115) 1072958..1073377 + 420 WP_235082372.1 hypothetical protein -
FS596_RS05060 (FS596_05120) 1073412..1074755 - 1344 WP_191976756.1 NCS2 family permease -
FS596_RS05065 (FS596_05125) 1074825..1076612 - 1788 WP_191976757.1 adenine deaminase C-terminal domain-containing protein -
FS596_RS05070 (FS596_05130) 1076750..1077682 + 933 WP_054305425.1 LysR family transcriptional regulator -
FS596_RS05075 (FS596_05135) 1077763..1077966 - 204 WP_015671220.1 HHA domain-containing protein Toxin
FS596_RS05080 (FS596_05140) 1078026..1078394 - 369 WP_061324804.1 Hha toxicity modulator TomB Antitoxin
FS596_RS05085 (FS596_05145) 1078751..1079104 - 354 WP_054305423.1 hypothetical protein -
FS596_RS05090 1079269..1079418 + 150 WP_191977214.1 hypothetical protein -
FS596_RS05095 (FS596_05150) 1079415..1080119 + 705 WP_212602155.1 ABC transporter ATP-binding protein -
FS596_RS05100 (FS596_05155) 1080116..1080982 + 867 WP_054305421.1 metal ABC transporter permease -
FS596_RS05105 (FS596_05160) 1080998..1081873 + 876 WP_128144334.1 metal ABC transporter substrate-binding protein -
FS596_RS05110 (FS596_05165) 1081972..1082112 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
FS596_RS05115 (FS596_05170) 1082122..1082376 - 255 WP_235082373.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8049.33 Da        Isoelectric Point: 7.9816

>T135543 WP_015671220.1 NZ_CP042353:c1077966-1077763 [Serratia rubidaea]
MTKTDYLMRLRKCTTIDTLERVIEKNKYALSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T135543 NZ_CP042353:c1077966-1077763 [Serratia rubidaea]
ATGACAAAAACCGACTACCTGATGCGTTTACGTAAATGCACCACTATCGATACGCTTGAAAGAGTGATTGAAAAAAACAA
GTACGCGCTCTCTGACGATGAGCTCGAGCTGTTTTACTCCGCAGCCGACCACCGTCTGGCTGAACTCACCATGAATAAGC
TGTATGACAAAATCCCGACGTCTGTTTGGAAATATGTAAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14393.20 Da        Isoelectric Point: 4.7790

>AT135543 WP_061324804.1 NZ_CP042353:c1078394-1078026 [Serratia rubidaea]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSYKIKYMDESDFSELVEEYL
DDTYTLFSNYGINDSDLRRWQKTKRRLFRMFSGEVCCTKMKT

Download         Length: 369 bp

>AT135543 NZ_CP042353:c1078394-1078026 [Serratia rubidaea]
ATGGATGAGTACTCGCCGAAACGGCATGATATTGCTCAACTCAAATTCTTGTGTGAAAATCTGTACGATGAAGGCATTGC
GACGTTGGGCGACAGCCATCACGGCTGGGTTAACGATCCGACTTCGGCCGTCAATCTGCAGCTGAATGAGCTGATCGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATAAAATACATGGACGAAAGTGACTTTTCAGAATTAGTCGAAGAGTACCTC
GACGATACCTATACGTTATTTAGTAACTACGGTATTAACGATTCCGATCTGCGTCGTTGGCAAAAAACTAAACGGCGACT
ATTCAGAATGTTCTCAGGAGAGGTTTGCTGTACGAAAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A857EAE6

Antitoxin

Source ID Structure
AlphaFold DB A0A140F0B9

References