Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4561391..4561967 Replicon chromosome
Accession NZ_CP042352
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag FS836_RS22275 Protein ID WP_001297643.1
Coordinates 4561391..4561678 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag FS836_RS22280 Protein ID WP_000063156.1
Coordinates 4561665..4561967 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS22255 (4557644) 4557644..4558924 - 1281 WP_001037380.1 DUF445 domain-containing protein -
FS836_RS22260 (4558965) 4558965..4560197 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
FS836_RS22265 (4560343) 4560343..4560438 - 96 Protein_4239 dienelactone hydrolase family protein -
FS836_RS22270 (4560495) 4560495..4561192 + 698 Protein_4240 IS1 family transposase -
FS836_RS22275 (4561391) 4561391..4561678 + 288 WP_001297643.1 BrnT family toxin Toxin
FS836_RS22280 (4561665) 4561665..4561967 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
FS836_RS22285 (4562001) 4562001..4562957 - 957 WP_001297640.1 GTPase -
FS836_RS22290 (4562968) 4562968..4563171 - 204 WP_000467859.1 YbdD/YjiX family protein -
FS836_RS22295 (4563221) 4563221..4565371 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T135541 WP_001297643.1 NZ_CP042352:4561391-4561678 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T135541 NZ_CP042352:4561391-4561678 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT135541 WP_000063156.1 NZ_CP042352:4561665-4561967 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT135541 NZ_CP042352:4561665-4561967 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References