Detailed information of TA system TA135540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4524777..4525480
• Replicon: chromosome
• Accession: NZ_CP042352
• Organism: Escherichia coli strain RM9513

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A853JWF2
• Locus tag: FS836_RS22090
• Protein ID: WP_032267594.1
• Coordinates: 4525139..4525480 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: FS836_RS22085
• Protein ID: WP_032267593.1
• Coordinates: 4524777..4525118 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS836_RS22040 (4520035)	4520035..4520670	+	636	WP_032267586.1	hypothetical protein	-
FS836_RS22045 (4520709)	4520709..4521164	+	456	WP_057688286.1	hypothetical protein	-
FS836_RS22050 (4521161)	4521161..4521295	+	135	WP_255380262.1	hypothetical protein	-
FS836_RS22055 (4521334)	4521334..4521621	+	288	WP_235839820.1	hypothetical protein	-
FS836_RS22060 (4521688)	4521688..4522227	+	540	WP_032267588.1	DUF4339 domain-containing protein	-
FS836_RS22065 (4522322)	4522322..4522795	+	474	WP_032267589.1	hypothetical protein	-
FS836_RS22070 (4522916)	4522916..4523737	+	822	WP_032267590.1	DUF932 domain-containing protein	-
FS836_RS22075 (4523768)	4523768..4524211	+	444	WP_032267591.1	antirestriction protein	-
FS836_RS22080 (4524224)	4524224..4524766	+	543	WP_032267592.1	DNA repair protein RadC	-
FS836_RS22085 (4524777)	4524777..4525118	+	342	WP_032267593.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FS836_RS22090 (4525139)	4525139..4525480	+	342	WP_032267594.1	TA system toxin CbtA family protein	Toxin
FS836_RS22095 (4525731)	4525731..4526738	+	1008	WP_032267595.1	restriction endonuclease	-
FS836_RS22100 (4527422)	4527422..4527565	-	144	Protein_4206	HNH endonuclease	-
FS836_RS22105 (4527702)	4527702..4528352	+	651	WP_001037966.1	HNH endonuclease	-
FS836_RS22110 (4528360)	4528360..4529340	-	981	WP_001295601.1	sialate O-acetylesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	4505882..4534376	28494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12767.68 Da
• Isoelectric Point: 6.8699

>T135540 WP_032267594.1 NZ_CP042352:4525139-4525480 [Escherichia coli]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDEKVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
GWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T135540 NZ_CP042352:4525139-4525480 [Escherichia coli]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
TCGCCTGCTGGAACAACATTATGGCCTGACGCTAAACGACACGCCGTTCAGTGATGAAAAGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GGCTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACAGGTTTATTGAAAGCGCG
GCACGGTGAACATTGGGAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12760.46 Da
• Isoelectric Point: 6.6213

>AT135540 WP_032267593.1 NZ_CP042352:4524777-4525118 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDLAFPHFISQMESMLTTGEINPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYLTYR

Nucleotide

• Length: 342 bp

>AT135540 NZ_CP042352:4524777-4525118 [Escherichia coli]
ATGAAATCTACTGATTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAATCGACTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATTTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATAAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCTCACTTATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A853JWF2