Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3035188..3035842 Replicon chromosome
Accession NZ_CP042352
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FS836_RS14975 Protein ID WP_000244777.1
Coordinates 3035188..3035595 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FS836_RS14980 Protein ID WP_000354046.1
Coordinates 3035576..3035842 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS14955 (3031145) 3031145..3032878 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
FS836_RS14960 (3032884) 3032884..3033594 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FS836_RS14965 (3033619) 3033619..3034515 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FS836_RS14970 (3034627) 3034627..3035148 + 522 WP_001055874.1 flavodoxin FldB -
FS836_RS14975 (3035188) 3035188..3035595 - 408 WP_000244777.1 protein YgfX Toxin
FS836_RS14980 (3035576) 3035576..3035842 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FS836_RS14985 (3036085) 3036085..3037065 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FS836_RS14990 (3037261) 3037261..3037920 - 660 WP_000250274.1 hemolysin III family protein -
FS836_RS14995 (3038084) 3038084..3038395 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FS836_RS15000 (3038440) 3038440..3039873 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
FS836_RS15005 (3039930) 3039930..3040673 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T135535 WP_000244777.1 NZ_CP042352:c3035595-3035188 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T135535 NZ_CP042352:c3035595-3035188 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT135535 WP_000354046.1 NZ_CP042352:c3035842-3035576 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT135535 NZ_CP042352:c3035842-3035576 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References