Detailed information of TA system TA135532

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2083274..2084109
• Replicon: chromosome
• Accession: NZ_CP042352
• Organism: Escherichia coli strain RM9513

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FS836_RS10490
• Protein ID: WP_064221650.1
• Coordinates: 2083732..2084109 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FS836_RS10485
• Protein ID: WP_141085548.1
• Coordinates: 2083274..2083642 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS836_RS10460 (2080389)	2080389..2080584	+	196	Protein_1948	DUF905 family protein	-
FS836_RS10465 (2080702)	2080702..2081520	+	819	WP_020239663.1	DUF932 domain-containing protein	-
FS836_RS10470 (2081862)	2081862..2082335	+	474	WP_001765426.1	antirestriction protein	-
FS836_RS10475 (2082351)	2082351..2082827	+	477	WP_001186775.1	RadC family protein	-
FS836_RS10480 (2082890)	2082890..2083111	+	222	WP_000692345.1	DUF987 domain-containing protein	-
FS836_RS10485 (2083274)	2083274..2083642	+	369	WP_141085548.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FS836_RS10490 (2083732)	2083732..2084109	+	378	WP_064221650.1	TA system toxin CbtA family protein	Toxin
FS836_RS10495 (2084106)	2084106..2084255	+	150	Protein_1955	DUF5983 family protein	-
FS836_RS10500 (2084355)	2084355..2084435	+	81	Protein_1956	hypothetical protein	-
FS836_RS10505 (2085233)	2085233..2085622	+	390	WP_077875581.1	transposase	-
FS836_RS10510 (2085893)	2085893..2086258	+	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
FS836_RS10515 (2086359)	2086359..2086688	-	330	WP_000450409.1	DUF496 family protein	-
FS836_RS10520 (2086860)	2086860..2087918	-	1059	WP_001200895.1	FUSC family protein	-
FS836_RS10525 (2088116)	2088116..2088589	-	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14150.14 Da
• Isoelectric Point: 7.3249

>T135532 WP_064221650.1 NZ_CP042352:2083732-2084109 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTQLARLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T135532 NZ_CP042352:2083732-2084109 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACAGCTCGC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13480.26 Da
• Isoelectric Point: 5.9541

>AT135532 WP_141085548.1 NZ_CP042352:2083274-2083642 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT135532 NZ_CP042352:2083274-2083642 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA