Detailed information of TA system TA135522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1536151..1536789
• Replicon: chromosome
• Accession: NZ_CP042352
• Organism: Escherichia coli strain RM9513

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FS836_RS07825
• Protein ID: WP_000813794.1
• Coordinates: 1536151..1536327 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FS836_RS07830
• Protein ID: WP_001270286.1
• Coordinates: 1536373..1536789 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS836_RS07805 (1531770)	1531770..1532984	-	1215	WP_001326689.1	BenE family transporter YdcO	-
FS836_RS07810 (1533037)	1533037..1533573	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FS836_RS07815 (1533646)	1533646..1535607	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FS836_RS07820 (1535699)	1535699..1535929	-	231	WP_000494244.1	YncJ family protein	-
FS836_RS07825 (1536151)	1536151..1536327	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FS836_RS07830 (1536373)	1536373..1536789	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FS836_RS07835 (1536868)	1536868..1538274	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FS836_RS07840 (1538519)	1538519..1539664	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FS836_RS07845 (1539682)	1539682..1540695	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
FS836_RS07850 (1540696)	1540696..1541637	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T135522 WP_000813794.1 NZ_CP042352:1536151-1536327 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T135522 NZ_CP042352:1536151-1536327 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT135522 WP_001270286.1 NZ_CP042352:1536373-1536789 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT135522 NZ_CP042352:1536373-1536789 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB