Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 521242..521860 Replicon chromosome
Accession NZ_CP042352
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FS836_RS02950 Protein ID WP_001291435.1
Coordinates 521242..521460 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FS836_RS02955 Protein ID WP_000344800.1
Coordinates 521486..521860 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS02915 (516531) 516531..517103 + 573 WP_000779831.1 YbaY family lipoprotein -
FS836_RS02920 (517134) 517134..517445 - 312 WP_000409911.1 MGMT family protein -
FS836_RS02930 (517824) 517824..518177 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FS836_RS02935 (518219) 518219..519769 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FS836_RS02940 (519933) 519933..520403 - 471 WP_000136192.1 YlaC family protein -
FS836_RS02945 (520519) 520519..521070 - 552 WP_000102564.1 maltose O-acetyltransferase -
FS836_RS02950 (521242) 521242..521460 - 219 WP_001291435.1 HHA domain-containing protein Toxin
FS836_RS02955 (521486) 521486..521860 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FS836_RS02960 (522406) 522406..525555 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FS836_RS02965 (525578) 525578..526771 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135517 WP_001291435.1 NZ_CP042352:c521460-521242 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T135517 NZ_CP042352:c521460-521242 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT135517 WP_000344800.1 NZ_CP042352:c521860-521486 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT135517 NZ_CP042352:c521860-521486 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References