Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 78303..78828 Replicon plasmid pRM9513
Accession NZ_CP042351
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FS836_RS00400 Protein ID WP_001159868.1
Coordinates 78523..78828 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FS836_RS00395 Protein ID WP_000813634.1
Coordinates 78303..78521 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS00365 (73713) 73713..74453 - 741 WP_000933099.1 abortive infection family protein -
FS836_RS00370 (74489) 74489..75070 - 582 WP_225395751.1 DUF2971 domain-containing protein -
FS836_RS00375 (75086) 75086..75277 - 192 Protein_74 hypothetical protein -
FS836_RS00380 (75274) 75274..75894 - 621 WP_001248529.1 hypothetical protein -
FS836_RS00385 (75891) 75891..76574 - 684 WP_010891291.1 DNA-binding protein -
FS836_RS00390 (76811) 76811..77973 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
FS836_RS00395 (78303) 78303..78521 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FS836_RS00400 (78523) 78523..78828 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FS836_RS00405 (78829) 78829..79635 + 807 WP_024243979.1 site-specific integrase -
FS836_RS00410 (79682) 79682..80326 + 645 WP_001477618.1 replication initiation protein RepE -
FS836_RS00415 (80914) 80914..82080 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FS836_RS00420 (82080) 82080..83051 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..93878 93878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T135514 WP_001159868.1 NZ_CP042351:78523-78828 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T135514 NZ_CP042351:78523-78828 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT135514 WP_000813634.1 NZ_CP042351:78303-78521 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT135514 NZ_CP042351:78303-78521 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References