Detailed information of TA system TA135513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 39118..39893
• Replicon: plasmid pRM9513
• Accession: NZ_CP042351
• Organism: Escherichia coli strain RM9513

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A6D0V6Q2
• Locus tag: FS836_RS00240
• Protein ID: WP_000405252.1
• Coordinates: 39411..39893 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FS836_RS00235
• Protein ID: WP_001346256.1
• Coordinates: 39118..39420 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS836_RS00210 (36265)	36265..37011	+	747	WP_130091596.1	conjugal transfer pilus acetylase TraX	-
FS836_RS00215 (37066)	37066..37626	+	561	WP_096977265.1	fertility inhibition protein FinO	-
FS836_RS00220 (37756)	37756..37967	+	212	Protein_43	hypothetical protein	-
FS836_RS00225 (38213)	38213..38281	+	69	Protein_44	endonuclease	-
- (38543)	38543..38602	-	60	NuclAT_1	-	-
- (38543)	38543..38602	-	60	NuclAT_1	-	-
- (38543)	38543..38602	-	60	NuclAT_1	-	-
- (38543)	38543..38602	-	60	NuclAT_1	-	-
FS836_RS00230 (38647)	38647..38796	+	150	WP_001312851.1	Hok/Gef family protein	-
FS836_RS00235 (39118)	39118..39420	+	303	WP_001346256.1	DUF1778 domain-containing protein	Antitoxin
FS836_RS00240 (39411)	39411..39893	+	483	WP_000405252.1	GNAT family N-acetyltransferase	Toxin
FS836_RS00245 (40203)	40203..40460	+	258	WP_000083826.1	replication regulatory protein RepA	-
FS836_RS00250 (40496)	40496..40729	-	234	WP_226058127.1	replication protein RepA	-
FS836_RS00255 (40720)	40720..40767	+	48	WP_235246954.1	hypothetical protein	-
FS836_RS00260 (40760)	40760..41617	+	858	WP_140436363.1	incFII family plasmid replication initiator RepA	-
FS836_RS00265 (42592)	42592..42831	+	240	WP_155849733.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..93878	93878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17687.47 Da
• Isoelectric Point: 7.1036

>T135513 WP_000405252.1 NZ_CP042351:39411-39893 [Escherichia coli]
MEINVTAPVLLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T135513 NZ_CP042351:39411-39893 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGTATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11602.19 Da
• Isoelectric Point: 4.4569

>AT135513 WP_001346256.1 NZ_CP042351:39118-39420 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT135513 NZ_CP042351:39118-39420 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCCGTTATTGATTATGCCGCTAGTCTGGTCAATAAGAACCGTACTGATTTCATCATTGAGCTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A6D0V6Q2