Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3365942..3366596 Replicon chromosome
Accession NZ_CP042350
Organism Escherichia coli strain RM10410

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FS611_RS16925 Protein ID WP_000244777.1
Coordinates 3365942..3366349 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FS611_RS16930 Protein ID WP_000354046.1
Coordinates 3366330..3366596 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS611_RS16905 3361899..3363632 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FS611_RS16910 3363638..3364348 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FS611_RS16915 3364373..3365269 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FS611_RS16920 3365381..3365902 + 522 WP_001055874.1 flavodoxin FldB -
FS611_RS16925 3365942..3366349 - 408 WP_000244777.1 toxin CptA Toxin
FS611_RS16930 3366330..3366596 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FS611_RS16935 3366839..3367819 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FS611_RS16940 3368015..3368674 - 660 WP_000250274.1 hemolysin III family protein -
FS611_RS16945 3368838..3369149 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FS611_RS16950 3369194..3370627 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FS611_RS16955 3370684..3371427 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T135503 WP_000244777.1 NZ_CP042350:c3366349-3365942 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T135503 NZ_CP042350:c3366349-3365942 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT135503 WP_000354046.1 NZ_CP042350:c3366596-3366330 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT135503 NZ_CP042350:c3366596-3366330 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References