Detailed information of TA system TA135492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1767140..1767666
• Replicon: chromosome
• Accession: NZ_CP042350
• Organism: Escherichia coli strain RM10410

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FS611_RS08845
• Protein ID: WP_000323025.1
• Coordinates: 1767140..1767427 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FS611_RS08850
• Protein ID: WP_000534858.1
• Coordinates: 1767427..1767666 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FS611_RS08805	1762299..1762511	+	213	WP_000087756.1	cold shock-like protein CspF	-
FS611_RS08810	1762566..1762655	+	90	WP_120795389.1	hypothetical protein	-
FS611_RS08815	1762933..1763685	-	753	WP_001047135.1	antitermination protein	-
FS611_RS08820	1763699..1764748	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
FS611_RS08825	1764750..1765028	-	279	WP_012304870.1	hypothetical protein	-
FS611_RS08830	1765095..1765346	-	252	WP_000980994.1	hypothetical protein	-
FS611_RS08835	1765606..1766718	+	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
FS611_RS08840	1766913..1767068	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
FS611_RS08845	1767140..1767427	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FS611_RS08850	1767427..1767666	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FS611_RS08855	1767691..1767963	+	273	WP_072033201.1	hypothetical protein	-
FS611_RS08860	1768005..1769233	+	1229	WP_088895425.1	IS3-like element IS2 family transposase	-
FS611_RS08865	1769535..1769867	+	333	WP_001301033.1	protein FlxA	-
FS611_RS08870	1770304..1771617	-	1314	WP_000589005.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1747836..1786464	38628
	-	inside	Prophage	-	stxB / stxA	1750486..1866092	115606
	-	inside	IScluster/Tn	-	-	1765606..1777993	12387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T135492 WP_000323025.1 NZ_CP042350:c1767427-1767140 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T135492 NZ_CP042350:c1767427-1767140 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT135492 WP_000534858.1 NZ_CP042350:c1767666-1767427 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT135492 NZ_CP042350:c1767666-1767427 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8