Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 136036..136561 Replicon plasmid pCTXM-GZ04
Accession NZ_CP042337
Organism Escherichia coli strain GZ04-0086

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag E3B71_RS26465 Protein ID WP_001159871.1
Coordinates 136036..136341 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag E3B71_RS26470 Protein ID WP_000813630.1
Coordinates 136343..136561 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3B71_RS26450 131998..133164 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
E3B71_RS26455 133752..134507 - 756 WP_000852146.1 replication initiation protein RepE -
E3B71_RS26460 135229..136035 - 807 WP_000016970.1 site-specific integrase -
E3B71_RS26465 136036..136341 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
E3B71_RS26470 136343..136561 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E3B71_RS26480 137121..137351 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
E3B71_RS26485 137348..137764 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
E3B71_RS26490 137839..139404 + 1566 WP_001128474.1 AAA family ATPase -
E3B71_RS26495 139389..140411 + 1023 WP_000361402.1 helicase UvrD -
E3B71_RS26500 140665..141351 - 687 Protein_170 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD iutA / iucD / iucC / iucB / iucA 1..159610 159610
- flank IS/Tn - - 140665..141012 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T135437 WP_001159871.1 NZ_CP042337:c136341-136036 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T135437 NZ_CP042337:c136341-136036 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT135437 WP_000813630.1 NZ_CP042337:c136561-136343 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT135437 NZ_CP042337:c136561-136343 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References