Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3620856..3621451 Replicon chromosome
Accession NZ_CP042336
Organism Escherichia coli strain GZ04-0086

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag E3B71_RS19720 Protein ID WP_000239579.1
Coordinates 3621101..3621451 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag E3B71_RS19715 Protein ID WP_001223208.1
Coordinates 3620856..3621107 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3B71_RS19705 3616520..3620299 + 3780 WP_000060921.1 autotransporter assembly complex protein TamB -
E3B71_RS19710 3620302..3620643 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
E3B71_RS19715 3620856..3621107 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
E3B71_RS19720 3621101..3621451 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
E3B71_RS19725 3621531..3622061 - 531 WP_000055072.1 inorganic diphosphatase -
E3B71_RS19730 3622371..3623327 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
E3B71_RS19735 3623637..3625139 + 1503 WP_000205791.1 sugar ABC transporter ATP-binding protein -
E3B71_RS19740 3625153..3626175 + 1023 WP_024198507.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T135423 WP_000239579.1 NZ_CP042336:3621101-3621451 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T135423 NZ_CP042336:3621101-3621451 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT135423 WP_001223208.1 NZ_CP042336:3620856-3621107 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT135423 NZ_CP042336:3620856-3621107 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References