Detailed information of TA system TA135407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 759640..760166
• Replicon: chromosome
• Accession: NZ_CP042336
• Organism: Escherichia coli strain GZ04-0086

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: E3B71_RS05695
• Protein ID: WP_000323025.1
• Coordinates: 759640..759927 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: E3B71_RS05700
• Protein ID: WP_000534858.1
• Coordinates: 759927..760166 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3B71_RS05650	754664..754879	-	216	WP_000839590.1	phage lysis protein EssD	-
E3B71_RS05655	755633..755848	-	216	WP_000066484.1	cold shock-like protein CspB	-
E3B71_RS05660	756149..756361	+	213	WP_000087756.1	cold shock-like protein CspF	-
E3B71_RS05665	756416..756505	+	90	WP_120795389.1	hypothetical protein	-
E3B71_RS05670	756783..757535	-	753	WP_001047135.1	antitermination protein	-
E3B71_RS05675	757549..758598	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
E3B71_RS05680	758600..758878	-	279	WP_012304870.1	hypothetical protein	-
E3B71_RS05685	758945..759196	-	252	WP_000980994.1	hypothetical protein	-
E3B71_RS05690	759413..759568	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
E3B71_RS05695	759640..759927	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
E3B71_RS05700	759927..760166	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
E3B71_RS05705	760191..760496	+	306	WP_001326990.1	hypothetical protein	-
E3B71_RS05710	760699..761031	+	333	WP_001301033.1	protein FlxA	-
E3B71_RS05715	761468..762781	-	1314	WP_000589005.1	ISNCY family transposase	-
E3B71_RS27720	763550..763837	-	288	Protein_754	hypothetical protein	-
E3B71_RS05730	764059..764463	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
E3B71_RS05735	764460..764807	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	722900..775746	52846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T135407 WP_000323025.1 NZ_CP042336:c759927-759640 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T135407 NZ_CP042336:c759927-759640 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT135407 WP_000534858.1 NZ_CP042336:c760166-759927 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT135407 NZ_CP042336:c760166-759927 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8