Detailed information of TA system TA135398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3164450..3164921
• Replicon: chromosome
• Accession: NZ_CP042326
• Organism: Euhalothece natronophila Z-M001

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FRE64_RS15585
• Protein ID: WP_146297072.1
• Coordinates: 3164450..3164710 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FRE64_RS15590
• Protein ID: WP_146297073.1
• Coordinates: 3164700..3164921 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRE64_RS15545	3159631..3160098	-	468	WP_146297064.1	Hsp20/alpha crystallin family protein	-
FRE64_RS15550	3160195..3160446	-	252	WP_146297065.1	hypothetical protein	-
FRE64_RS15555	3160589..3161218	+	630	WP_146297066.1	phosphoribosylglycinamide formyltransferase	-
FRE64_RS15560	3161255..3161719	+	465	WP_146297067.1	NADAR family protein	-
FRE64_RS15565	3161870..3162490	-	621	WP_146297068.1	cyanoexosortase A system-associated protein	-
FRE64_RS15570	3162515..3162751	-	237	WP_146297069.1	hypothetical protein	-
FRE64_RS15575	3162771..3163862	+	1092	WP_146297070.1	ISAs1 family transposase	-
FRE64_RS15580	3164182..3164394	+	213	WP_146297071.1	hypothetical protein	-
FRE64_RS15585	3164450..3164710	+	261	WP_146297072.1	BrnT family toxin	Toxin
FRE64_RS15590	3164700..3164921	+	222	WP_146297073.1	CopG family transcriptional regulator	Antitoxin
FRE64_RS15595	3165086..3166177	+	1092	WP_146297074.1	ISAs1 family transposase	-
FRE64_RS15600	3166258..3166704	-	447	WP_146297395.1	hypothetical protein	-
FRE64_RS15605	3166707..3166943	-	237	WP_146297075.1	hypothetical protein	-
FRE64_RS15610	3167704..3168057	-	354	WP_186708878.1	DUF559 domain-containing protein	-
FRE64_RS15615	3168143..3168515	-	373	Protein_3081	type II toxin-antitoxin system VapC family toxin	-
FRE64_RS15620	3168512..3168751	-	240	WP_146297077.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FRE64_RS15625	3168846..3169181	-	336	WP_146297078.1	DUF86 domain-containing protein	-
FRE64_RS15630	3169178..3169480	-	303	WP_146297397.1	nucleotidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10212.63 Da
• Isoelectric Point: 10.0937

>T135398 WP_146297072.1 NZ_CP042326:3164450-3164710 [Euhalothece natronophila Z-M001]
VKFTYDPAKSKSNQSKHGINFEQAQQLWLDPLRVEIEAQSTTEPRFMIIGKIEGKYWSAIVTYRHQTIRIISVRRSRQEE
IRVYES

Nucleotide

• Length: 261 bp

>T135398 NZ_CP042326:3164450-3164710 [Euhalothece natronophila Z-M001]
GTGAAATTTACATATGATCCTGCGAAAAGTAAAAGTAATCAGTCTAAACATGGAATTAACTTTGAACAAGCGCAACAACT
TTGGTTAGATCCACTTCGGGTAGAAATTGAAGCCCAATCAACCACAGAGCCTCGATTTATGATTATTGGTAAAATTGAGG
GGAAATACTGGTCTGCAATTGTTACTTATCGTCATCAAACAATTCGCATCATTTCGGTAAGAAGATCGCGTCAAGAGGAG
ATAAGAGTATATGAATCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8549.82 Da
• Isoelectric Point: 4.6437

>AT135398 WP_146297073.1 NZ_CP042326:3164700-3164921 [Euhalothece natronophila Z-M001]
MNPEELDKKFDAGEDVMEFFDLSTLKRPELESKPINVNFPQWMIKGLDEEAKRLGVNREAVIKTWIAQKLDQK

Nucleotide

• Length: 222 bp

>AT135398 NZ_CP042326:3164700-3164921 [Euhalothece natronophila Z-M001]
ATGAATCCTGAAGAATTAGATAAGAAATTTGATGCTGGTGAAGATGTAATGGAATTTTTTGATTTATCCACTCTTAAACG
CCCTGAGTTAGAGAGCAAGCCAATTAATGTTAACTTTCCGCAATGGATGATTAAAGGATTAGATGAAGAAGCAAAACGAT
TGGGAGTTAATCGAGAAGCCGTTATTAAAACTTGGATCGCGCAAAAACTAGATCAAAAATAG