Detailed information of TA system TA135396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2917389..2917935
• Replicon: chromosome
• Accession: NZ_CP042326
• Organism: Euhalothece natronophila Z-M001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FRE64_RS14375
• Protein ID: WP_146296860.1
• Coordinates: 2917389..2917724 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FRE64_RS14380
• Protein ID: WP_146296861.1
• Coordinates: 2917711..2917935 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRE64_RS14350	2912452..2912820	+	369	WP_146296856.1	four helix bundle protein	-
FRE64_RS14355	2912852..2913343	-	492	WP_146296857.1	WbuC family cupin fold metalloprotein	-
FRE64_RS14360	2913458..2915455	+	1998	WP_146296858.1	excinuclease ABC subunit UvrB	-
FRE64_RS14365	2915644..2916063	+	420	WP_146297377.1	hypothetical protein	-
FRE64_RS14370	2916120..2917400	+	1281	WP_146296859.1	ATP-binding protein	-
FRE64_RS14375	2917389..2917724	-	336	WP_146296860.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FRE64_RS14380	2917711..2917935	-	225	WP_146296861.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
FRE64_RS14385	2918142..2918612	+	471	WP_146296862.1	helix-turn-helix domain-containing protein	-
FRE64_RS14390	2918618..2919190	+	573	WP_146296863.1	PIN domain-containing protein	-
FRE64_RS14395	2919210..2919428	-	219	Protein_2842	hypothetical protein	-
FRE64_RS14400	2919371..2919580	-	210	WP_186708854.1	IS1 family transposase	-
FRE64_RS14405	2919609..2919872	-	264	WP_186708856.1	hypothetical protein	-
FRE64_RS14410	2919962..2920429	-	468	WP_146296865.1	chemotaxis protein CheW	-
FRE64_RS14415	2920526..2920825	-	300	WP_146296866.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-
FRE64_RS14420	2921122..2921964	+	843	WP_146296867.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

• Antitoxin ParD (3-54)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12195.28 Da
• Isoelectric Point: 10.2971

>T135396 WP_146296860.1 NZ_CP042326:c2917724-2917389 [Euhalothece natronophila Z-M001]
IKRGEIYYAALDPVIGSESAKRCPVLIVSNDINNRVAATVTILPLTSQVSKIYPFEVFLPPEKSGLPKPSKVQAQQIRTI
SKQRLQGNQIGKLEPSLMQSVDATIKLHLSL

Nucleotide

• Length: 336 bp

>T135396 NZ_CP042326:c2917724-2917389 [Euhalothece natronophila Z-M001]
ATAAAACGTGGTGAAATTTACTATGCTGCCCTCGATCCTGTCATTGGTTCAGAATCAGCTAAACGCTGCCCCGTATTGAT
TGTAAGCAATGATATTAACAATCGAGTCGCGGCTACTGTGACTATTTTGCCCCTAACTTCACAAGTAAGCAAAATCTATC
CCTTTGAAGTGTTTTTACCTCCTGAAAAAAGTGGTTTACCTAAACCTTCTAAAGTCCAAGCCCAGCAAATTAGAACCATC
TCAAAACAGCGGCTTCAAGGCAATCAAATTGGAAAATTAGAACCATCTCTAATGCAATCAGTTGATGCAACGATTAAATT
ACATCTATCTCTGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8326.24 Da
• Isoelectric Point: 4.0745

>AT135396 WP_146296861.1 NZ_CP042326:c2917935-2917711 [Euhalothece natronophila Z-M001]
MESQKLSISLSPEMVAFIEDYQQKTRCQSRSQVIAEALSLLKTQELEKAYQEASAELDEAWENTAGDGLGDKTW

Nucleotide

• Length: 225 bp

>AT135396 NZ_CP042326:c2917935-2917711 [Euhalothece natronophila Z-M001]
ATGGAATCGCAAAAACTTTCTATTTCCTTATCCCCAGAGATGGTTGCTTTTATCGAGGATTATCAGCAGAAAACTCGGTG
CCAATCTCGTTCTCAGGTTATTGCGGAAGCACTCTCCTTATTAAAAACGCAGGAACTGGAAAAAGCGTACCAAGAAGCCT
CTGCTGAACTCGATGAAGCATGGGAAAATACAGCAGGAGATGGGTTAGGGGATAAAACGTGGTGA