Detailed information of TA system TA135395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2034251..2034829
• Replicon: chromosome
• Accession: NZ_CP042326
• Organism: Euhalothece natronophila Z-M001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FRE64_RS09880
• Protein ID: WP_146295913.1
• Coordinates: 2034251..2034583 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FRE64_RS09885
• Protein ID: WP_146295914.1
• Coordinates: 2034590..2034829 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRE64_RS09840	2029288..2029728	+	441	WP_146295905.1	protein tyrosine phosphatase family protein	-
FRE64_RS09845	2029712..2030107	-	396	WP_146295906.1	transcriptional repressor	-
FRE64_RS09850	2030184..2030480	+	297	WP_186708761.1	phosphoribosylformylglycinamidine synthase subunit PurS	-
FRE64_RS09855	2030484..2031179	+	696	WP_146295908.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
FRE64_RS09860	2031284..2032111	-	828	WP_146295909.1	class I SAM-dependent methyltransferase	-
FRE64_RS09865	2032124..2032663	-	540	WP_146295910.1	bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR	-
FRE64_RS09870	2032680..2033165	-	486	WP_146295911.1	DUF2062 domain-containing protein	-
FRE64_RS09875	2033311..2034243	+	933	WP_146295912.1	NAD-dependent epimerase/dehydratase family protein	-
FRE64_RS09880	2034251..2034583	-	333	WP_146295913.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FRE64_RS09885	2034590..2034829	-	240	WP_146295914.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FRE64_RS09890	2034950..2036572	-	1623	WP_146295915.1	chaperonin GroEL	-
FRE64_RS09895	2036609..2036920	-	312	WP_146295916.1	co-chaperone GroES	-
FRE64_RS09900	2037085..2037495	+	411	WP_146295917.1	ferredoxin	-
FRE64_RS09905	2037580..2038239	+	660	WP_146295918.1	response regulator transcription factor	-
FRE64_RS09910	2038228..2039517	-	1290	WP_146295919.1	O-antigen ligase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-106)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12456.52 Da
• Isoelectric Point: 9.5820

>T135395 WP_146295913.1 NZ_CP042326:c2034583-2034251 [Euhalothece natronophila Z-M001]
MYIPARGDFVHLNLNPRTGREQCGERFGLIISPQKFNKISSLAFTCPITTKSKGFSLEVKIRDNPGRVYGVVLVHHLKSI
DWKARNVQYIEDAPSAVIEEVMAKLEPLIF

Nucleotide

• Length: 333 bp

>T135395 NZ_CP042326:c2034583-2034251 [Euhalothece natronophila Z-M001]
ATGTATATTCCAGCTCGTGGAGATTTTGTTCATCTAAACCTCAACCCCCGCACTGGACGAGAGCAATGCGGGGAGCGTTT
TGGGCTTATTATTTCGCCTCAAAAGTTCAATAAAATTTCTTCCTTAGCTTTTACCTGCCCAATTACCACAAAAAGTAAAG
GTTTTTCTTTAGAAGTGAAAATTAGAGATAACCCTGGGAGAGTTTATGGAGTTGTCTTAGTTCACCACCTCAAAAGTATC
GACTGGAAAGCAAGAAATGTGCAATACATTGAAGATGCTCCATCAGCTGTCATAGAAGAGGTAATGGCAAAACTAGAACC
TTTAATTTTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8753.84 Da
• Isoelectric Point: 4.3118

>AT135395 WP_146295914.1 NZ_CP042326:c2034829-2034590 [Euhalothece natronophila Z-M001]
MTTSVRKWGNSLAVRIPQPIAEQANLKEGVNVSFTVSEEGSIILSPKPKYTLDELLEGVSPEHFEGETEWGEPVGKEVW

Nucleotide

• Length: 240 bp

>AT135395 NZ_CP042326:c2034829-2034590 [Euhalothece natronophila Z-M001]
ATGACAACCTCAGTAAGAAAATGGGGCAATAGTTTAGCAGTTCGGATTCCGCAACCGATCGCGGAACAGGCTAATTTAAA
AGAAGGCGTTAATGTATCTTTTACTGTTTCTGAGGAAGGATCAATTATTCTGAGTCCCAAGCCCAAATATACTTTGGATG
AATTACTCGAAGGGGTTTCCCCAGAACATTTTGAAGGAGAGACAGAATGGGGAGAACCAGTTGGTAAAGAGGTCTGGTAG