Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2180282..2180901 Replicon chromosome
Accession NZ_CP042319
Organism Klebsiella quasipneumoniae strain 68-17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FLM29_RS10535 Protein ID WP_002892050.1
Coordinates 2180282..2180500 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FLM29_RS10540 Protein ID WP_002892066.1
Coordinates 2180527..2180901 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLM29_RS10500 2176330..2176593 + 264 WP_004204754.1 type B 50S ribosomal protein L31 -
FLM29_RS10505 2176593..2176733 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FLM29_RS10510 2176730..2177428 - 699 WP_179417059.1 GNAT family N-acetyltransferase -
FLM29_RS10515 2177529..2178986 + 1458 WP_179417060.1 PLP-dependent aminotransferase family protein -
FLM29_RS10520 2178955..2179425 - 471 WP_049093338.1 YlaC family protein -
FLM29_RS10525 2179446..2179586 + 141 WP_179417094.1 hypothetical protein -
FLM29_RS10530 2179558..2180124 - 567 WP_087832569.1 maltose O-acetyltransferase -
FLM29_RS10535 2180282..2180500 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FLM29_RS10540 2180527..2180901 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FLM29_RS10545 2181386..2184532 - 3147 WP_017898892.1 multidrug efflux RND transporter permease subunit -
FLM29_RS10550 2184555..2185748 - 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135346 WP_002892050.1 NZ_CP042319:c2180500-2180282 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T135346 NZ_CP042319:c2180500-2180282 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCACCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT135346 WP_002892066.1 NZ_CP042319:c2180901-2180527 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT135346 NZ_CP042319:c2180901-2180527 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References