Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1221269..1221894 Replicon chromosome
Accession NZ_CP042318
Organism Klebsiella pneumoniae strain 46-16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FLM28_RS06085 Protein ID WP_002882817.1
Coordinates 1221511..1221894 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FLM28_RS06080 Protein ID WP_004150355.1
Coordinates 1221269..1221511 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLM28_RS06055 1217260..1217859 + 600 WP_004151865.1 glucose-1-phosphatase -
FLM28_RS06060 1217853..1218713 + 861 WP_002882807.1 virulence factor BrkB family protein -
FLM28_RS06065 1218710..1219147 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FLM28_RS06070 1219192..1220133 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FLM28_RS06075 1220147..1221064 - 918 WP_002882812.1 alpha/beta hydrolase -
FLM28_RS06080 1221269..1221511 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FLM28_RS06085 1221511..1221894 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FLM28_RS06090 1222068..1222997 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FLM28_RS06095 1222994..1223629 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FLM28_RS06100 1223626..1224528 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T135331 WP_002882817.1 NZ_CP042318:1221511-1221894 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T135331 NZ_CP042318:1221511-1221894 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT135331 WP_004150355.1 NZ_CP042318:1221269-1221511 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT135331 NZ_CP042318:1221269-1221511 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References