Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2307029..2307648 Replicon chromosome
Accession NZ_CP042315
Organism Klebsiella pneumoniae strain 89-17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FLM33_RS11250 Protein ID WP_002892050.1
Coordinates 2307029..2307247 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FLM33_RS11255 Protein ID WP_002892066.1
Coordinates 2307274..2307648 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLM33_RS11215 2303076..2303339 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
FLM33_RS11220 2303339..2303479 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FLM33_RS11225 2303476..2304174 - 699 WP_032435564.1 GNAT family N-acetyltransferase -
FLM33_RS11230 2304275..2305726 + 1452 WP_032435563.1 PLP-dependent aminotransferase family protein -
FLM33_RS11235 2305701..2306171 - 471 WP_002892026.1 YlaC family protein -
FLM33_RS11240 2306192..2306332 + 141 WP_004147370.1 hypothetical protein -
FLM33_RS11245 2306304..2306870 - 567 WP_002892030.1 maltose O-acetyltransferase -
FLM33_RS11250 2307029..2307247 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FLM33_RS11255 2307274..2307648 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FLM33_RS11260 2308134..2311280 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
FLM33_RS11265 2311303..2312496 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135300 WP_002892050.1 NZ_CP042315:c2307247-2307029 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T135300 NZ_CP042315:c2307247-2307029 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT135300 WP_002892066.1 NZ_CP042315:c2307648-2307274 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT135300 NZ_CP042315:c2307648-2307274 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References