Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2418271..2418890 Replicon chromosome
Accession NZ_CP042309
Organism Klebsiella pneumoniae strain 109-17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FLM40_RS11815 Protein ID WP_002892050.1
Coordinates 2418271..2418489 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FLM40_RS11820 Protein ID WP_002892066.1
Coordinates 2418516..2418890 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLM40_RS11780 2414318..2414581 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
FLM40_RS11785 2414581..2414721 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FLM40_RS11790 2414718..2415416 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
FLM40_RS11795 2415517..2416968 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FLM40_RS11800 2416943..2417413 - 471 WP_002892026.1 YlaC family protein -
FLM40_RS11805 2417434..2417574 + 141 WP_004147370.1 hypothetical protein -
FLM40_RS11810 2417546..2418112 - 567 WP_002892030.1 maltose O-acetyltransferase -
FLM40_RS11815 2418271..2418489 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FLM40_RS11820 2418516..2418890 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FLM40_RS11825 2419376..2422522 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
FLM40_RS11830 2422545..2423738 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135230 WP_002892050.1 NZ_CP042309:c2418489-2418271 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T135230 NZ_CP042309:c2418489-2418271 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT135230 WP_002892066.1 NZ_CP042309:c2418890-2418516 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT135230 NZ_CP042309:c2418890-2418516 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References