Detailed information of TA system TA135212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 1991261..1991985
• Replicon: chromosome
• Accession: NZ_CP042303
• Organism: Paracidovorax citrulli strain NWB SC107

Toxin (Protein)

• Gene name: avrRxo1
• Uniprot ID: -
• Locus tag: FRC90_RS09270
• Protein ID: WP_233423719.1
• Coordinates: 1991572..1991985 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: arc1
• Uniprot ID: A1TRP1
• Locus tag: FRC90_RS09265
• Protein ID: WP_011796139.1
• Coordinates: 1991261..1991557 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRC90_RS09255 (FRC90_09245)	1989441..1990238	+	798	WP_011796141.1	BMA_0021/BMA_0022 family TOMM bacteriocin	-
FRC90_RS09260 (FRC90_09250)	1990248..1991102	+	855	WP_232521745.1	class I SAM-dependent methyltransferase	-
FRC90_RS09265 (FRC90_09255)	1991261..1991557	-	297	WP_011796139.1	hypothetical protein	Antitoxin
FRC90_RS09270 (FRC90_09260)	1991572..1991985	-	414	WP_233423719.1	hypothetical protein	Toxin
FRC90_RS09275 (FRC90_09265)	1992001..1992813	+	813	WP_011793283.1	IS5 family transposase	-
FRC90_RS09280	1992820..1993371	-	552	WP_233427208.1	XopAJ/AvrRxo1 family type III secretion system effector zeta toxin	-
FRC90_RS09285	1993469..1993738	+	270	WP_225981105.1	hypothetical protein	-
FRC90_RS09290 (FRC90_09275)	1993952..1996156	+	2205	WP_041828059.1	TOMM precursor leader peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1992001..1992813	812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14544.26 Da
• Isoelectric Point: 6.3243

>T135212 WP_233423719.1 NZ_CP042303:c1991985-1991572 [Paracidovorax citrulli]
MTSTPRSKVHNTLYVAVANGFSAVRGHRLDVIDKFLSNPTLGSYHLFGTTGNGSKAMVASVVNGELSIHDPQLYEAITDP
QGARAGDSGDQIIDAALIDRLTKGIDDPARAMDARTALERYAGMTWSDALAAHSSLT

Nucleotide

• Length: 414 bp

>T135212 NZ_CP042303:c1991985-1991572 [Paracidovorax citrulli]
TTGACTTCCACTCCGAGGAGCAAAGTTCATAACACGCTCTACGTTGCGGTTGCCAACGGATTCTCGGCGGTTCGCGGGCA
CCGGCTCGATGTCATCGACAAGTTTCTCTCGAACCCGACCTTGGGGAGCTATCATCTATTCGGCACTACCGGGAATGGCA
GCAAGGCGATGGTTGCCAGCGTCGTCAACGGCGAACTGAGCATTCATGACCCTCAGTTATACGAGGCCATTACAGATCCC
CAAGGTGCCCGGGCAGGCGATAGCGGCGATCAGATCATTGACGCTGCGCTGATCGACCGGCTGACCAAAGGTATAGACGA
CCCAGCGCGAGCCATGGATGCCCGCACCGCATTGGAGCGGTACGCGGGCATGACGTGGTCGGACGCTCTGGCCGCCCATA
GTTCACTGACTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11106.62 Da
• Isoelectric Point: 6.9795

>AT135212 WP_011796139.1 NZ_CP042303:c1991557-1991261 [Paracidovorax citrulli]
MTGLKGADALDFHKKLKERNAALRNAELNAAKELADKNGKEPFDLVKLERLCDTSQAGRLVEGTERQAMYEYMYYVEYKN
VGTLQDFARVVTTLASWS

Nucleotide

• Length: 297 bp

>AT135212 NZ_CP042303:c1991557-1991261 [Paracidovorax citrulli]
ATGACAGGCCTGAAAGGCGCAGATGCGCTCGACTTCCATAAGAAACTCAAGGAACGCAATGCTGCTTTACGCAATGCGGA
GTTGAACGCGGCGAAGGAACTGGCCGACAAAAATGGCAAGGAGCCTTTTGATCTCGTGAAACTGGAGAGGCTTTGCGATA
CCAGTCAAGCAGGCCGCCTTGTTGAAGGGACCGAACGGCAAGCCATGTACGAATACATGTACTACGTCGAGTACAAGAAT
GTTGGCACGTTGCAGGATTTCGCCCGGGTGGTGACAACGCTCGCGTCCTGGTCTTAA

Structures

Antitoxin

• AlphaFold DB: A1TRP1