Detailed information of TA system TA135210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/-
• Location: 2062558..2063792
• Replicon: chromosome
• Accession: NZ_CP042302
• Organism: Paracidovorax citrulli strain NWB SC074

Toxin (Protein)

• Gene name: avrRxo1
• Uniprot ID: -
• Locus tag: FRC75_RS09280
• Protein ID: WP_232521744.1
• Coordinates: 2062558..2063481 (+)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: arc1
• Uniprot ID: A1TRP1
• Locus tag: FRC75_RS09285
• Protein ID: WP_011796139.1
• Coordinates: 2063496..2063792 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRC75_RS09260 (FRC75_09275)	2058131..2058469	-	339	WP_011796135.1	hypothetical protein	-
FRC75_RS24530	2058491..2058619	-	129	WP_258868820.1	hypothetical protein	-
FRC75_RS09265 (FRC75_09280)	2058785..2059708	-	924	WP_228193810.1	FHA domain-containing protein	-
FRC75_RS09270 (FRC75_09285)	2059773..2061977	-	2205	WP_041828059.1	TOMM precursor leader peptide-binding protein	-
FRC75_RS09275	2062191..2062460	-	270	WP_225981105.1	hypothetical protein	-
FRC75_RS09280 (FRC75_09290)	2062558..2063481	+	924	WP_232521744.1	XopAJ/AvrRxo1 family type III secretion system effector zeta toxin	Toxin
FRC75_RS09285 (FRC75_09295)	2063496..2063792	+	297	WP_011796139.1	hypothetical protein	Antitoxin
FRC75_RS09290 (FRC75_09300)	2063951..2064805	-	855	WP_232521745.1	class I SAM-dependent methyltransferase	-
FRC75_RS09295 (FRC75_09305)	2064815..2065612	-	798	WP_011796141.1	BMA_0021/BMA_0022 family TOMM bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (38-177)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 32876.16 Da
• Isoelectric Point: 6.2340

>T135210 WP_232521744.1 NZ_CP042302:2062558-2063481 [Paracidovorax citrulli]
MAHPQNWRPERRQLHDRLIGDAKTAAQDFAEVIERGGHPPTLFALRGNTAAGKTRMATQTIPVLANALKESGGAGCINPD
IFKRSLAETPEGAKLTSAQVHAESCILADRLEDELRLQKTASGAAISMLVDKRLAGAHEIDAYIKLAQETGRKIELCDID
APLEQSLMGVLQRKPEGDAPRPPYVAVANGFSAVRGHRLDVIDKFLSNPTLGSYHLFGTTGNGSKAMVASVVNGELSIHD
PQLYEAITDPQGARAGDSGDQIIDAALIDRLTKGIDDPARAMDARTALERYAGMTWSDALAAHSSLT

Nucleotide

• Length: 924 bp

>T135210 NZ_CP042302:2062558-2063481 [Paracidovorax citrulli]
TTGGCCCATCCTCAAAACTGGCGCCCCGAGCGGCGTCAATTGCATGACCGGCTGATCGGCGATGCGAAAACTGCAGCCCA
GGATTTCGCCGAGGTGATCGAGCGCGGTGGCCACCCACCGACATTGTTCGCGTTGCGAGGCAATACGGCTGCGGGAAAGA
CACGCATGGCGACACAAACCATCCCCGTACTCGCCAATGCCCTCAAGGAGAGTGGCGGCGCCGGCTGTATTAATCCCGAC
ATCTTCAAACGTTCGCTCGCAGAAACTCCAGAGGGAGCAAAACTTACCTCGGCGCAGGTACACGCCGAGTCGTGCATCCT
CGCTGACCGGCTTGAAGACGAACTCCGATTGCAGAAGACCGCGAGCGGCGCGGCGATCAGCATGCTGGTCGATAAGCGAC
TGGCTGGAGCGCACGAAATCGACGCGTACATCAAGCTGGCTCAGGAGACCGGTCGCAAAATCGAGTTGTGCGATATTGAT
GCTCCACTCGAGCAGTCACTCATGGGCGTGCTGCAGCGCAAGCCCGAAGGTGACGCCCCCCGGCCACCCTACGTTGCGGT
TGCCAACGGATTCTCGGCGGTTCGCGGGCACCGGCTCGATGTCATCGACAAGTTTCTCTCGAACCCGACCTTGGGGAGCT
ATCATCTATTCGGCACTACCGGGAATGGCAGCAAGGCGATGGTTGCCAGCGTCGTCAACGGCGAACTGAGCATTCATGAC
CCTCAGTTATACGAGGCCATTACAGATCCCCAAGGTGCCCGGGCAGGCGATAGCGGCGATCAGATCATTGACGCTGCGCT
GATCGACCGGCTGACCAAAGGTATAGACGACCCAGCGCGAGCCATGGATGCCCGCACCGCATTGGAGCGGTACGCGGGCA
TGACGTGGTCGGACGCTCTGGCCGCCCATAGTTCACTGACTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11106.62 Da
• Isoelectric Point: 6.9795

>AT135210 WP_011796139.1 NZ_CP042302:2063496-2063792 [Paracidovorax citrulli]
MTGLKGADALDFHKKLKERNAALRNAELNAAKELADKNGKEPFDLVKLERLCDTSQAGRLVEGTERQAMYEYMYYVEYKN
VGTLQDFARVVTTLASWS

Nucleotide

• Length: 297 bp

>AT135210 NZ_CP042302:2063496-2063792 [Paracidovorax citrulli]
ATGACAGGCCTGAAAGGCGCAGATGCGCTCGACTTCCATAAGAAACTCAAGGAACGCAATGCTGCTTTACGCAATGCGGA
GTTGAACGCGGCGAAGGAACTGGCCGACAAAAATGGCAAGGAGCCTTTTGATCTCGTGAAACTGGAGAGGCTTTGCGATA
CCAGTCAAGCAGGCCGCCTTGTTGAAGGGACCGAACGGCAAGCCATGTACGAATACATGTACTACGTCGAGTACAAGAAT
GTTGGCACGTTGCAGGATTTCGCCCGGGTGGTGACAACGCTCGCGTCCTGGTCTTAA

Structures

Antitoxin

• AlphaFold DB: A1TRP1