Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4581877..4582502 Replicon chromosome
Accession NZ_CP042298
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name vapC Uniprot ID A0A4C4KA40
Locus tag FRV13_RS24685 Protein ID WP_047084715.1
Coordinates 4582104..4582502 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FRV13_RS24680 Protein ID WP_000450524.1
Coordinates 4581877..4582104 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS24655 4577679..4578149 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FRV13_RS24660 4578149..4578721 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FRV13_RS24665 4578867..4579745 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FRV13_RS24670 4579762..4580796 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FRV13_RS24675 4581009..4581722 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FRV13_RS24680 4581877..4582104 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FRV13_RS24685 4582104..4582502 + 399 WP_047084715.1 tRNA(fMet)-specific endonuclease VapC Toxin
FRV13_RS24690 4582649..4583512 + 864 WP_047084716.1 neutral zinc metallopeptidase -
FRV13_RS24695 4583527..4585542 + 2016 WP_047084717.1 tRNA cytosine(34) acetyltransferase TmcA -
FRV13_RS24700 4585616..4586314 + 699 WP_000679812.1 esterase -
FRV13_RS24705 4586424..4586624 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14866.19 Da        Isoelectric Point: 8.5325

>T135189 WP_047084715.1 NZ_CP042298:4582104-4582502 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELAQQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T135189 NZ_CP042298:4582104-4582502 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCAGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT135189 WP_000450524.1 NZ_CP042298:4581877-4582104 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT135189 NZ_CP042298:4581877-4582104 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4C4KA40

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References