Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2599889..2600386 Replicon chromosome
Accession NZ_CP042298
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FRV13_RS14875 Protein ID WP_001173343.1
Coordinates 2599889..2600062 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A4C4HZ80
Locus tag FRV13_RS14880 Protein ID WP_072057953.1
Coordinates 2600090..2600386 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS14865 2596560..2598017 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FRV13_RS14870 2598253..2599770 + 1518 WP_001295074.1 lysine--tRNA ligase -
FRV13_RS14875 2599889..2600062 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FRV13_RS14880 2600090..2600386 - 297 WP_072057953.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FRV13_RS14885 2600613..2600885 - 273 WP_000405647.1 N-acetyltransferase -
FRV13_RS14890 2600897..2601127 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
FRV13_RS14895 2601308..2602939 + 1632 WP_001216484.1 sensor histidine kinase -
FRV13_RS14900 2602936..2603655 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T135184 WP_001173343.1 NZ_CP042298:c2600062-2599889 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T135184 NZ_CP042298:c2600062-2599889 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11451.97 Da        Isoelectric Point: 4.1705

>AT135184 WP_072057953.1 NZ_CP042298:c2600386-2600090 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRALMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT135184 NZ_CP042298:c2600386-2600090 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGAGCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A4C4HZ80

References