Detailed information of TA system TA135183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2496691..2497286
• Replicon: chromosome
• Accession: NZ_CP042298
• Organism: Escherichia coli strain RM9088

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: FRV13_RS14345
• Protein ID: WP_000239581.1
• Coordinates: 2496691..2497041 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: FRV13_RS14350
• Protein ID: WP_001223213.1
• Coordinates: 2497035..2497286 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRV13_RS14325	2492013..2493035	-	1023	WP_047084900.1	ABC transporter permease	-
FRV13_RS14330	2493049..2494551	-	1503	WP_047084901.1	sugar ABC transporter ATP-binding protein	-
FRV13_RS14335	2494691..2495647	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FRV13_RS14340	2495957..2496487	+	531	WP_000055075.1	inorganic diphosphatase	-
FRV13_RS14345	2496691..2497041	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
FRV13_RS14350	2497035..2497286	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FRV13_RS14355	2497498..2497839	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FRV13_RS14360	2497842..2501621	-	3780	WP_047084902.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T135183 WP_000239581.1 NZ_CP042298:c2497041-2496691 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T135183 NZ_CP042298:c2497041-2496691 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT135183 WP_001223213.1 NZ_CP042298:c2497286-2497035 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT135183 NZ_CP042298:c2497286-2497035 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCTAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7