Detailed information of TA system TA135167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 363713..365411
• Replicon: chromosome
• Accession: NZ_CP042298
• Organism: Escherichia coli strain RM9088

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A3Y1KBU9
• Locus tag: FRV13_RS03305
• Protein ID: WP_047085429.1
• Coordinates: 364482..365411 (+)
• Length: 310 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: FRV13_RS03300
• Protein ID: WP_047085427.1
• Coordinates: 363713..364495 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRV13_RS03295	360331..363429	-	3099	WP_042003137.1	type I restriction endonuclease subunit R	-
FRV13_RS03300	363713..364495	+	783	WP_047085427.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
FRV13_RS03305	364482..365411	+	930	WP_047085429.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FRV13_RS03315	365808..366707	-	900	WP_000687182.1	LysR family transcriptional regulator	-
FRV13_RS03320	366806..367528	+	723	WP_000361975.1	SDR family oxidoreductase	-
FRV13_RS03325	367692..367970	+	279	WP_077790490.1	hypothetical protein	-
FRV13_RS03335	368672..369556	-	885	WP_001119037.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 310 a.a.
• Molecular weight: 34960.38 Da
• Isoelectric Point: 7.3232

>T135167 WP_047085429.1 NZ_CP042298:364482-365411 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNSK
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMSVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKRNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVSKLERVLEQYS

Nucleotide

• Length: 930 bp

>T135167 NZ_CP042298:364482-365411 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCTATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATTACCGCGATCCGACAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCAGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGTCGGTGTGCGAACGTGTGGAAATGGAGT
ATGGTTTCACCACTATGAGTGTCGTTTCTCTGGCGGATCTTTATGGCGGTAAAATCTGCGCGGCGCTTGATCGTCAACAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTCCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGTGAGTTCTTGCTCAGTTTTAAGCGAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTAAAAGATAAAAATCCTGCGAAGT
ATGTGGCGGCGGTGAGTAAGCTGGAGAGGGTTCTGGAGCAGTATTCTTAG

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29193.40 Da
• Isoelectric Point: 8.5995

>AT135167 WP_047085427.1 NZ_CP042298:363713-364495 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGRPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTPFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGSGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT135167 NZ_CP042298:363713-364495 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAAAATGGCTGGCTCAGGCGCATCGCTCACGGCGTGTACTGCCGTACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTGCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTACAGGGCCATGCGCACTATACCGAACCGGGCAGGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTAA
CGCCATTTACCACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGCCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTCTATCTTGCCCGGCGTAATGGACATCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTTCGGGAAAGCGGCAAATCATACCCGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3Y1KBU9