Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 154520..155079 Replicon plasmid p1RM9088
Accession NZ_CP042296
Organism Escherichia coli strain RM9088

Toxin (Protein)


Gene name relE Uniprot ID A0A0B1JMW5
Locus tag FRV13_RS00905 Protein ID WP_000421249.1
Coordinates 154520..154795 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B1JM87
Locus tag FRV13_RS00910 Protein ID WP_001355337.1
Coordinates 154795..155079 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRV13_RS00870 150213..150959 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
FRV13_RS00875 151045..151494 - 450 Protein_160 DNA methylase -
FRV13_RS00880 151573..151874 - 302 Protein_161 hypothetical protein -
FRV13_RS00885 152012..152974 + 963 WP_000959884.1 plasmid segregation protein ParM -
FRV13_RS00890 152977..153327 + 351 WP_024166183.1 plasmid partitioning/stability family protein -
FRV13_RS00895 153434..153907 + 474 Protein_164 hypothetical protein -
FRV13_RS00900 154133..154414 - 282 WP_001105063.1 hypothetical protein -
FRV13_RS00905 154520..154795 - 276 WP_000421249.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FRV13_RS00910 154795..155079 - 285 WP_001355337.1 ribbon-helix-helix protein, CopG family Antitoxin
FRV13_RS28160 155418..155627 + 210 WP_157839880.1 hypothetical protein -
FRV13_RS00920 155800..156085 + 286 Protein_169 hypothetical protein -
FRV13_RS00930 157912..158784 + 873 Protein_170 IS3 family transposase -
FRV13_RS00935 159040..159417 - 378 Protein_171 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyD / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..167256 167256
- flank IS/Tn - - 157912..158784 872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10722.42 Da        Isoelectric Point: 9.9324

>T135161 WP_000421249.1 NZ_CP042296:c154795-154520 [Escherichia coli]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T135161 NZ_CP042296:c154795-154520 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10550.99 Da        Isoelectric Point: 5.6706

>AT135161 WP_001355337.1 NZ_CP042296:c155079-154795 [Escherichia coli]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Download         Length: 285 bp

>AT135161 NZ_CP042296:c155079-154795 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B1JMW5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B1JM87

References