Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 59746..60839 Replicon plasmid pHDC14-2.133K
Accession NZ_CP042290
Organism Enterococcus hirae strain HDC14-2

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag FQ488_RS13900 Protein ID WP_185185109.1
Coordinates 59970..60839 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag FQ488_RS13895 Protein ID WP_185185110.1
Coordinates 59746..59955 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQ488_RS13870 55248..55499 + 252 WP_002294510.1 hypothetical protein -
FQ488_RS13875 55550..56008 - 459 WP_002325145.1 hypothetical protein -
FQ488_RS13880 56091..56654 + 564 Protein_64 recombinase zinc beta ribbon domain-containing protein -
FQ488_RS13885 57097..58581 + 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
FQ488_RS13890 58635..59438 + 804 WP_073285199.1 lincosamide nucleotidyltransferase Lnu(B) -
FQ488_RS13895 59746..59955 + 210 WP_185185110.1 helix-turn-helix transcriptional regulator Antitoxin
FQ488_RS13900 59970..60839 + 870 WP_185185109.1 nucleotidyltransferase domain-containing protein Toxin
FQ488_RS13905 60820..61554 + 735 WP_010725595.1 class I SAM-dependent methyltransferase -
FQ488_RS13910 61587..62495 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
FQ488_RS13915 62492..63034 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
FQ488_RS13920 63127..63921 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
FQ488_RS13925 64197..64769 + 573 WP_001079938.1 HTH domain-containing protein -
FQ488_RS13930 64742..65149 + 408 WP_072387101.1 WYL domain-containing protein -
FQ488_RS13935 65260..65472 + 213 Protein_75 riboflavin biosynthesis protein RibD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(2-52)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32950.68 Da        Isoelectric Point: 5.0947

>T135155 WP_185185109.1 NZ_CP042290:59970-60839 [Enterococcus hirae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVKEIATEINNFLNEENLNERKQI

Download         Length: 870 bp

>T135155 NZ_CP042290:59970-60839 [Enterococcus hirae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTATTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAAAGAAATTGCAACGGAGATAAATAACTTTTTAAACGAGGAGAATTTAAATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 7818.06 Da        Isoelectric Point: 4.7063

>AT135155 WP_185185110.1 NZ_CP042290:59746-59955 [Enterococcus hirae]
MIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 210 bp

>AT135155 NZ_CP042290:59746-59955 [Enterococcus hirae]
ATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCAGACTCCACAGAATTT
CAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAGGTGTCAAGTTTGTGC
AGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References