Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 366662..367233 Replicon chromosome
Accession NZ_CP042289
Organism Enterococcus hirae strain HDC14-2

Toxin (Protein)


Gene name mazF Uniprot ID I6SW09
Locus tag FQ488_RS01690 Protein ID WP_010738536.1
Coordinates 366662..367003 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I6T4M0
Locus tag FQ488_RS01695 Protein ID WP_010738535.1
Coordinates 367003..367233 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQ488_RS01670 361886..362887 + 1002 WP_034866081.1 aspartate-semialdehyde dehydrogenase -
FQ488_RS01675 362884..364233 + 1350 WP_010720195.1 aspartate kinase -
FQ488_RS01680 364193..365302 + 1110 WP_025480561.1 amidohydrolase -
FQ488_RS01685 365332..366498 + 1167 WP_185184869.1 pyridoxal phosphate-dependent aminotransferase -
FQ488_RS01690 366662..367003 - 342 WP_010738536.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FQ488_RS01695 367003..367233 - 231 WP_010738535.1 hypothetical protein Antitoxin
FQ488_RS01700 367693..369072 + 1380 WP_010738534.1 FAD-dependent oxidoreductase -
FQ488_RS01705 369253..370218 - 966 WP_034866085.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13101.46 Da        Isoelectric Point: 10.1962

>T135154 WP_010738536.1 NZ_CP042289:c367003-366662 [Enterococcus hirae]
VPKARNYIPKKGDIVWIDFDPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLISQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Download         Length: 342 bp

>T135154 NZ_CP042289:c367003-366662 [Enterococcus hirae]
GTGCCTAAAGCTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGAAAT
TCAAAAGAGAAGACCAGGCTTGGTTGTATCTCGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTGTCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8577.57 Da        Isoelectric Point: 4.1196

>AT135154 WP_010738535.1 NZ_CP042289:c367233-367003 [Enterococcus hirae]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Download         Length: 231 bp

>AT135154 NZ_CP042289:c367233-367003 [Enterococcus hirae]
ATGCTCATTACAAAATCTAGATTACAAGGTAGTTCTGTAGTAATTACACTACCGTCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9DJ27

Antitoxin

Source ID Structure
AlphaFold DB A0A449E900

References