Detailed information of TA system TA135143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 5115409..5116017
• Replicon: chromosome
• Accession: NZ_CP042272
• Organism: Paenibacillus polymyxa strain ZF197

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: FQU75_RS23145
• Protein ID: WP_007429335.1
• Coordinates: 5115667..5116017 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FQU75_RS23140
• Protein ID: WP_187360601.1
• Coordinates: 5115409..5115660 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU75_RS23120	5111806..5111991	-	186	Protein_4463	helix-turn-helix transcriptional regulator	-
FQU75_RS23125	5111994..5112233	+	240	Protein_4464	lysine transporter LysE	-
FQU75_RS23130	5112344..5113621	+	1278	WP_149035786.1	outer membrane lipoprotein carrier protein LolA	-
FQU75_RS23135	5113889..5115085	+	1197	WP_149036213.1	alanine racemase	-
FQU75_RS23140	5115409..5115660	+	252	WP_187360601.1	ribbon-helix-helix protein, CopG family	Antitoxin
FQU75_RS23145	5115667..5116017	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FQU75_RS23150	5116181..5118403	+	2223	WP_187360602.1	RNA-binding transcriptional accessory protein	-
FQU75_RS23155	5118510..5118644	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
FQU75_RS23160	5118787..5119299	+	513	WP_149035787.1	SprT family protein	-
FQU75_RS23175	5119675..5120550	-	876	WP_149035788.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T135143 WP_007429335.1 NZ_CP042272:5115667-5116017 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T135143 NZ_CP042272:5115667-5116017 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGTCCTACTGTGATCGTAGCAGCTATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAGATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAGATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCACT
ACAAATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9495.88 Da
• Isoelectric Point: 6.4671

>AT135143 WP_187360601.1 NZ_CP042272:5115409-5115660 [Paenibacillus polymyxa]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVSERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT135143 NZ_CP042272:5115409-5115660 [Paenibacillus polymyxa]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAAAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAAGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAGA
TGGCTAAAATTAACTTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1