Detailed information of TA system TA135140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3496326..3497304
• Replicon: chromosome
• Accession: NZ_CP042270
• Organism: Bacillus thuringiensis strain GA-A07

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: FQZ25_RS18265
• Protein ID: WP_000624977.1
• Coordinates: 3496326..3497063 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: FQZ25_RS18270
• Protein ID: WP_000237819.1
• Coordinates: 3497176..3497304 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQZ25_RS18240	3491939..3492346	+	408	WP_000072495.1	VOC family protein	-
FQZ25_RS18245	3492370..3492750	+	381	WP_000497668.1	methyltransferase	-
FQZ25_RS18250	3492908..3494593	-	1686	WP_152305379.1	alpha-keto acid decarboxylase family protein	-
FQZ25_RS18255	3494701..3495183	+	483	WP_000191885.1	MarR family winged helix-turn-helix transcriptional regulator	-
FQZ25_RS18260	3495350..3496087	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FQZ25_RS18265	3496326..3497063	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
FQZ25_RS18270	3497176..3497304	+	129	WP_000237819.1	hypothetical protein	Antitoxin
FQZ25_RS18275	3497377..3497553	+	177	WP_000852629.1	stage II sporulation protein SB	-
FQZ25_RS18280	3497569..3497958	-	390	WP_000713869.1	YxeA family protein	-
FQZ25_RS18285	3498170..3499639	+	1470	WP_000287546.1	beta-Ala-His dipeptidase	-
FQZ25_RS18290	3499885..3500694	+	810	WP_000664544.1	C39 family peptidase	-
FQZ25_RS18295	3500720..3501322	+	603	WP_000517259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T135140 WP_000624977.1 NZ_CP042270:3496326-3497063 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T135140 NZ_CP042270:3496326-3497063 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT135140 WP_000237819.1 NZ_CP042270:3497176-3497304 [Bacillus thuringiensis]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT135140 NZ_CP042270:3497176-3497304 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2