Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1444256..1444898 Replicon chromosome
Accession NZ_CP042270
Organism Bacillus thuringiensis strain GA-A07

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FQZ25_RS07705 Protein ID WP_000635963.1
Coordinates 1444548..1444898 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FQZ25_RS07700 Protein ID WP_000004570.1
Coordinates 1444256..1444543 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQZ25_RS07675 1439573..1440535 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
FQZ25_RS07680 1440528..1441100 - 573 WP_000586917.1 rhomboid family intramembrane serine protease -
FQZ25_RS07685 1441194..1441553 + 360 WP_000583416.1 holo-ACP synthase -
FQZ25_RS07690 1441710..1442660 + 951 WP_071740808.1 outer membrane lipoprotein carrier protein LolA -
FQZ25_RS07695 1442778..1443947 + 1170 WP_000390615.1 alanine racemase -
FQZ25_RS07700 1444256..1444543 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FQZ25_RS07705 1444548..1444898 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FQZ25_RS07710 1444966..1447134 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
FQZ25_RS07715 1447192..1447308 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FQZ25_RS07720 1447504..1447962 + 459 WP_071740807.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T135139 WP_000635963.1 NZ_CP042270:1444548-1444898 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T135139 NZ_CP042270:1444548-1444898 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT135139 WP_000004570.1 NZ_CP042270:1444256-1444543 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT135139 NZ_CP042270:1444256-1444543 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References