Detailed information of TA system TA135137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4585534..4586142
• Replicon: chromosome
• Accession: NZ_CP042269
• Organism: Pseudomonas aeruginosa strain HOU1

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q9I5J9
• Locus tag: FQ758_RS22540
• Protein ID: WP_003114156.1
• Coordinates: 4585534..4585881 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: FQ758_RS22545
• Protein ID: WP_034037999.1
• Coordinates: 4585891..4586142 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQ758_RS22510	4580893..4581165	+	273	WP_003120794.1	cysteine-rich CWC family protein	-
FQ758_RS22515	4581165..4581857	+	693	WP_003114159.1	16S rRNA pseudouridine(516) synthase	-
FQ758_RS22520	4581993..4583036	+	1044	WP_126577588.1	L,D-transpeptidase	-
FQ758_RS22525	4583116..4583853	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
FQ758_RS22530	4584305..4585207	+	903	WP_126577589.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
FQ758_RS22540	4585534..4585881	-	348	WP_003114156.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FQ758_RS22545	4585891..4586142	-	252	WP_034037999.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FQ758_RS22550	4586315..4587340	-	1026	WP_052189723.1	tyrosine-type recombinase/integrase	-
FQ758_RS22555	4587340..4588632	-	1293	WP_003115206.1	hypothetical protein	-
FQ758_RS22560	4588862..4590136	-	1275	WP_105412577.1	zonular occludens toxin family protein	-
FQ758_RS22565	4590140..4590496	-	357	WP_003114150.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4576430..4620948	44518

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12984.78 Da
• Isoelectric Point: 4.4212

>T135137 WP_003114156.1 NZ_CP042269:c4585881-4585534 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T135137 NZ_CP042269:c4585881-4585534 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9444.76 Da
• Isoelectric Point: 9.4808

>AT135137 WP_034037999.1 NZ_CP042269:c4586142-4585891 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSTDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT135137 NZ_CP042269:c4586142-4585891 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTACTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: Q9I5J9