Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 52834..53374 Replicon plasmid pB3526CHC_150k
Accession NZ_CP042253
Organism Legionella longbeachae strain B3526CHC

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag FQU71_RS00285 Protein ID WP_014845117.1
Coordinates 53054..53374 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag FQU71_RS00280 Protein ID WP_014845118.1
Coordinates 52834..53067 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQU71_RS00255 47963..50461 + 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -
FQU71_RS00260 50641..50922 - 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
FQU71_RS00265 50915..51175 - 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
FQU71_RS00270 51388..52272 + 885 WP_014845120.1 recombinase family protein -
FQU71_RS00275 52378..52725 + 348 WP_080031944.1 WGR domain-containing protein -
FQU71_RS00280 52834..53067 + 234 WP_014845118.1 hypothetical protein Antitoxin
FQU71_RS00285 53054..53374 + 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FQU71_RS00290 53812..54204 - 393 Protein_57 methyltransferase domain-containing protein -
FQU71_RS00295 54570..55103 - 534 WP_061637559.1 IS91 family transposase -
FQU71_RS00300 55125..55616 - 492 WP_051009158.1 IS91 family transposase -
FQU71_RS19000 55688..55843 + 156 WP_154080667.1 hypothetical protein -
FQU71_RS00305 56208..56795 + 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
FQU71_RS00310 56943..57434 + 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
FQU71_RS00315 58060..58362 + 303 WP_014845112.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-29 - 1..150432 150432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T135115 WP_014845117.1 NZ_CP042253:53054-53374 [Legionella longbeachae]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T135115 NZ_CP042253:53054-53374 [Legionella longbeachae]
ATGAATCGTGGTGATGTATATTGGGTAAACCTCGACCCTACTACAGGCTCTGAAATCAACAAACTCAGACCTTGCGTTTT
AGTTGGAGCAACACCGATTAATCAAGCAAGACGCACTGTTGTCGTCGTTCCTCTTTCAAGCTCTGCTACAGCCAGACCAC
CAATTACCATCACTGTTTCTTGTCTTGGCAAACAAGTTACCGCAGTTTGTGATCAAATAAGAACGATTGATAAAAGTCGT
TTAAAAAAAGTGGCAGGAAACCTTTCAGATAAAGATTTAAATGCCTTGGATGATGGGTTACGACAAGTTTTATGCCTTTG
A

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT135115 WP_014845118.1 NZ_CP042253:52834-53067 [Legionella longbeachae]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT135115 NZ_CP042253:52834-53067 [Legionella longbeachae]
ATGAATAAAGTTTTAATATCAATACCCGATCAAATCGCTTCTAGAATGAGAGCAGCAATACCTCAGCGACAACGCAGCAA
GGTGATTGCTCATCTTATTGAAAAAGAAATTGAACGACGTGAAAAAGCGCTCTATGAATGCGCTTTAGCTGTTGAAAATG
ATCATGGTTTACAAAATGAAATGAACGATTGGGATATAACGGTTCAAGATGGATTAACTGATGAATCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References