Detailed information of TA system TA135114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 50641..51175
• Replicon: plasmid pB3526CHC_150k
• Accession: NZ_CP042253
• Organism: Legionella longbeachae strain B3526CHC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7G4RL39
• Locus tag: FQU71_RS00260
• Protein ID: WP_014845122.1
• Coordinates: 50641..50922 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7G4RL40
• Locus tag: FQU71_RS00265
• Protein ID: WP_014845121.1
• Coordinates: 50915..51175 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU71_RS00235	46060..46383	+	324	WP_014845127.1	hypothetical protein	-
FQU71_RS00240	46436..47254	+	819	WP_014845126.1	hypothetical protein	-
FQU71_RS00245	47293..47646	+	354	WP_014845125.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FQU71_RS00250	47624..47914	+	291	WP_014845124.1	helix-turn-helix domain-containing protein	-
FQU71_RS00255	47963..50461	+	2499	WP_014845123.1	DEAD/DEAH box helicase family protein	-
FQU71_RS00260	50641..50922	-	282	WP_014845122.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FQU71_RS00265	50915..51175	-	261	WP_014845121.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FQU71_RS00270	51388..52272	+	885	WP_014845120.1	recombinase family protein	-
FQU71_RS00275	52378..52725	+	348	WP_080031944.1	WGR domain-containing protein	-
FQU71_RS00280	52834..53067	+	234	WP_014845118.1	hypothetical protein	-
FQU71_RS00285	53054..53374	+	321	WP_014845117.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FQU71_RS00290	53812..54204	-	393	Protein_57	methyltransferase domain-containing protein	-
FQU71_RS00295	54570..55103	-	534	WP_061637559.1	IS91 family transposase	-
FQU71_RS00300	55125..55616	-	492	WP_051009158.1	IS91 family transposase	-
FQU71_RS19000	55688..55843	+	156	WP_154080667.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..150432	150432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11028.69 Da
• Isoelectric Point: 8.9816

>T135114 WP_014845122.1 NZ_CP042253:c50922-50641 [Legionella longbeachae]
MLELELATQFKRDLKKITKQGKRRTLLDSIVEQLQKEEPLDPKHKDHSLSVNWDGYRECHITPDWLLIYKTIKDDRIQLL
RLSRTGSHSDLFG

Nucleotide

• Length: 282 bp

>T135114 NZ_CP042253:c50922-50641 [Legionella longbeachae]
ATGCTTGAATTAGAGTTGGCGACACAGTTTAAGCGTGATTTGAAAAAGATTACGAAGCAAGGGAAAAGAAGGACGCTCTT
AGACTCTATTGTAGAGCAATTGCAAAAGGAAGAACCCCTGGATCCAAAGCATAAGGATCACAGTCTTTCCGTAAATTGGG
ATGGTTATCGTGAATGTCACATTACCCCCGATTGGTTATTAATTTATAAGACCATTAAAGATGATCGCATACAATTGCTG
CGATTATCACGAACAGGATCACATAGTGATTTGTTTGGGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9594.08 Da
• Isoelectric Point: 6.9803

>AT135114 WP_014845121.1 NZ_CP042253:c51175-50915 [Legionella longbeachae]
MNKAATINARIEPALKMQAEAILHKVGLSTAEAIRLFYSQVCLQNGLPFEVKIPNKETREAMAELESGKGERFKTMKDVW
DSVDNA

Nucleotide

• Length: 261 bp

>AT135114 NZ_CP042253:c51175-50915 [Legionella longbeachae]
ATGAATAAGGCGGCAACAATTAATGCTCGTATTGAGCCTGCTCTTAAAATGCAGGCAGAAGCGATTTTGCATAAGGTAGG
CTTATCTACAGCCGAGGCGATTCGACTGTTCTATAGCCAGGTCTGTCTCCAAAATGGCCTGCCTTTTGAGGTAAAAATAC
CGAACAAAGAGACAAGAGAGGCCATGGCAGAGCTGGAATCAGGAAAAGGCGAGCGATTTAAAACAATGAAGGATGTATGG
GATTCTGTAGATAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A7G4RL39

Antitoxin

• AlphaFold DB: A0A7G4RL40