Detailed information of TA system TA135112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2154342..2154978
• Replicon: chromosome
• Accession: NZ_CP042252
• Organism: Bacillus licheniformis strain KNU11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: FQB22_RS10925
• Protein ID: WP_003179128.1
• Coordinates: 2154628..2154978 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: FQB22_RS10920
• Protein ID: WP_006638778.1
• Coordinates: 2154342..2154623 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQB22_RS10900	2149457..2150938	+	1482	WP_009330132.1	PH domain-containing protein	-
FQB22_RS10905	2150935..2151534	-	600	WP_003179118.1	rhomboid family intramembrane serine protease	-
FQB22_RS10910	2151877..2152836	+	960	WP_152847888.1	outer membrane lipoprotein carrier protein LolA	-
FQB22_RS10915	2153061..2154230	+	1170	WP_025807384.1	alanine racemase	-
FQB22_RS10920	2154342..2154623	+	282	WP_006638778.1	hypothetical protein	Antitoxin
FQB22_RS10925	2154628..2154978	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FQB22_RS10930	2155096..2155923	+	828	WP_003179130.1	RsbT co-antagonist protein RsbRA	-
FQB22_RS10935	2155927..2156292	+	366	WP_003179132.1	STAS domain-containing protein	-
FQB22_RS10940	2156295..2156696	+	402	WP_003179135.1	anti-sigma regulatory factor	-
FQB22_RS10945	2156707..2157714	+	1008	WP_003179137.1	PP2C family protein-serine/threonine phosphatase	-
FQB22_RS10950	2157773..2158099	+	327	WP_003179140.1	anti-sigma factor antagonist	-
FQB22_RS10955	2158099..2158584	+	486	WP_003179142.1	anti-sigma B factor RsbW	-
FQB22_RS10960	2158550..2159341	+	792	WP_003179144.1	RNA polymerase sigma factor SigB	-
FQB22_RS10965	2159338..2159937	+	600	WP_003179145.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T135112 WP_003179128.1 NZ_CP042252:2154628-2154978 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T135112 NZ_CP042252:2154628-2154978 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT135112 WP_006638778.1 NZ_CP042252:2154342-2154623 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT135112 NZ_CP042252:2154342-2154623 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2