Detailed information of TA system TA135110

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1068990..1069806
• Replicon: chromosome
• Accession: NZ_CP042251
• Organism: Geobacillus thermoleovorans strain ARTRW1

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: G8MYR9
• Locus tag: FP515_RS05650
• Protein ID: WP_014195290.1
• Coordinates: 1068990..1069409 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: G8MYS0
• Locus tag: FP515_RS05655
• Protein ID: WP_014195291.1
• Coordinates: 1069399..1069806 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FP515_RS05625	1065765..1066280	+	516	WP_014195285.1	GNAT family N-acetyltransferase	-
FP515_RS05630	1066352..1067623	+	1272	WP_122983902.1	DUF3533 domain-containing protein	-
FP515_RS05635	1067648..1067860	+	213	WP_014195287.1	ATP-binding cassette domain-containing protein	-
FP515_RS05640	1067827..1068297	+	471	Protein_1019	ABC transporter permease	-
FP515_RS05645	1068309..1068962	+	654	WP_014195289.1	TetR/AcrR family transcriptional regulator	-
FP515_RS05650	1068990..1069409	-	420	WP_014195290.1	DUF86 domain-containing protein	Toxin
FP515_RS05655	1069399..1069806	-	408	WP_014195291.1	nucleotidyltransferase domain-containing protein	Antitoxin
FP515_RS05660	1070087..1072219	-	2133	WP_014195292.1	ATP-dependent Clp protease ATP-binding subunit	-
FP515_RS05665	1072487..1073233	+	747	WP_025039183.1	FixH family protein	-
FP515_RS05670	1073341..1074381	+	1041	WP_014195294.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (9-98)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15907.43 Da
• Isoelectric Point: 8.2859

>T135110 WP_014195290.1 NZ_CP042251:c1069409-1068990 [Geobacillus thermoleovorans]
MKSDVILNKISVIERCLKRIREEYNGDPKNLQNYTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSISKKMKAMVGFRNIAVHDYQQLNLGILQAIVEHHLDDFKQFTKAILDYAKKNS

Nucleotide

• Length: 420 bp

>T135110 NZ_CP042251:c1069409-1068990 [Geobacillus thermoleovorans]
ATGAAAAGTGATGTCATTTTAAACAAGATCAGCGTGATCGAACGCTGCCTGAAACGAATTCGTGAAGAATATAACGGCGA
TCCAAAAAATTTACAAAATTACACAAAACAAGATTCGATCGTTCTGAATTTACAGCGGGCGTGCGAGGCATGCATCGATT
TGGCCATGCATATTGTGGCCGAGCAGAAATTCGGATTGCCGCAGCATAGCCGCGATGCATTCGCTCTCCTCGAAGAACAT
GGGGTCATCTCCCCTTCCATAAGCAAAAAGATGAAGGCGATGGTCGGATTCCGCAACATCGCCGTTCACGACTATCAACA
ACTGAACCTTGGCATCTTGCAAGCCATCGTCGAACACCATCTTGATGATTTCAAACAATTTACGAAAGCCATCCTCGATT
ATGCTAAGAAAAACAGCTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15273.46 Da
• Isoelectric Point: 4.8382

>AT135110 WP_014195291.1 NZ_CP042251:c1069806-1069399 [Geobacillus thermoleovorans]
MPNEMETIIIQTLRPALHPFVIYLFGSAARGTLRPDSDVDIAFVSDGEPHDPYELFRLAGELADKLGRDVDLVDLRQAST
VFQAQVVSTGKAIDCRDERKRAEFEMKTLKMYVKLNEERAPVLKQITESGSIYEK

Nucleotide

• Length: 408 bp

>AT135110 NZ_CP042251:c1069806-1069399 [Geobacillus thermoleovorans]
TTGCCAAACGAAATGGAGACGATCATCATTCAGACGCTCCGCCCGGCTCTTCACCCGTTCGTCATCTACCTGTTCGGTTC
AGCCGCCCGTGGGACGCTGCGCCCAGACAGCGATGTCGACATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATG
AGCTGTTTCGGCTCGCCGGGGAGTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACC
GTGTTTCAAGCGCAAGTCGTCTCGACGGGAAAAGCCATTGATTGCCGCGACGAGCGGAAACGGGCTGAGTTCGAAATGAA
AACATTGAAAATGTATGTGAAACTAAACGAAGAGAGAGCACCGGTGTTAAAACAAATTACGGAAAGCGGGAGCATATATG
AAAAGTGA

Structures

Toxin

• AlphaFold DB: G8MYR9

Antitoxin

• AlphaFold DB: A0A7U9EPL2