Detailed information of TA system TA135081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 20164..20665
• Replicon: plasmid pBCE049-3
• Accession: NZ_CP042249
• Organism: Escherichia coli strain BCE049

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: FQU84_RS01600
• Protein ID: WP_000702246.1
• Coordinates: 20405..20665 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: FQU84_RS01595
• Protein ID: WP_001132407.1
• Coordinates: 20164..20415 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU84_RS01540	15507..15956	+	450	WP_052974092.1	hypothetical protein	-
FQU84_RS01545	15962..16165	+	204	WP_000224735.1	hypothetical protein	-
FQU84_RS01550	16167..16345	+	179	Protein_19	hypothetical protein	-
FQU84_RS01560	16679..16999	+	321	WP_001401701.1	hypothetical protein	-
FQU84_RS01565	17130..17567	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
FQU84_RS01570	17592..17774	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
FQU84_RS01575	18072..18515	+	444	WP_145768465.1	hypothetical protein	-
FQU84_RS01580	18622..18888	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
FQU84_RS01585	19308..19568	-	261	WP_023225992.1	hypothetical protein	-
FQU84_RS01590	19896..20252	+	357	Protein_26	hypothetical protein	-
FQU84_RS01595	20164..20415	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
FQU84_RS01600	20405..20665	-	261	WP_000702246.1	hypothetical protein	Toxin
FQU84_RS01605	20717..20941	-	225	WP_000814384.1	hypothetical protein	-
FQU84_RS01610	21039..21248	-	210	WP_000866037.1	hypothetical protein	-
FQU84_RS01615	21289..21612	-	324	WP_000427402.1	hypothetical protein	-
FQU84_RS01625	22193..22522	-	330	WP_000855121.1	hypothetical protein	-
FQU84_RS01640	23122..23787	+	666	WP_000532148.1	P-loop NTPase	-
FQU84_RS27070	23861..24145	+	285	WP_001162371.1	hypothetical protein	-
FQU84_RS01650	24163..24759	-	597	WP_000125164.1	hypothetical protein	-
FQU84_RS01655	24909..25070	-	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..57435	57435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T135081 WP_000702246.1 NZ_CP042249:c20665-20405 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T135081 NZ_CP042249:c20665-20405 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT135081 WP_001132407.1 NZ_CP042249:c20415-20164 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT135081 NZ_CP042249:c20415-20164 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7