Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3741726..3742344 Replicon chromosome
Accession NZ_CP042246
Organism Escherichia coli strain PU-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FQU83_RS20500 Protein ID WP_001291435.1
Coordinates 3742126..3742344 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FQU83_RS20495 Protein ID WP_000344800.1
Coordinates 3741726..3742100 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FQU83_RS20485 3736816..3738009 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FQU83_RS20490 3738032..3741181 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FQU83_RS20495 3741726..3742100 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FQU83_RS20500 3742126..3742344 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FQU83_RS20505 3742518..3743069 + 552 WP_000102543.1 maltose O-acetyltransferase -
FQU83_RS20510 3743185..3743655 + 471 WP_000136192.1 YlaC family protein -
FQU83_RS20515 3743819..3745369 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FQU83_RS20520 3745411..3745764 - 354 WP_101356861.1 DUF1428 family protein -
FQU83_RS20530 3746143..3746454 + 312 WP_000409908.1 MGMT family protein -
FQU83_RS20535 3746485..3747057 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T135059 WP_001291435.1 NZ_CP042246:3742126-3742344 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T135059 NZ_CP042246:3742126-3742344 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT135059 WP_000344800.1 NZ_CP042246:3741726-3742100 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT135059 NZ_CP042246:3741726-3742100 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References