Detailed information of TA system TA135056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2557358..2558946
• Replicon: chromosome
• Accession: NZ_CP042246
• Organism: Escherichia coli strain PU-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: FQU83_RS14295
• Protein ID: WP_001125475.1
• Coordinates: 2557624..2558946 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FQU83_RS14290
• Protein ID: WP_001332145.1
• Coordinates: 2557358..2557624 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQU83_RS14275	2553726..2555177	+	1452	WP_000854637.1	tagaturonate reductase	-
FQU83_RS14280	2555374..2556288	+	915	WP_001306069.1	bestrophin family protein	-
FQU83_RS14285	2556292..2557050	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
FQU83_RS14290	2557358..2557624	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FQU83_RS14295	2557624..2558946	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FQU83_RS14305	2559205..2559462	-	258	WP_001210030.1	hypothetical protein	-
FQU83_RS14310	2559572..2560354	+	783	WP_001221834.1	SDR family oxidoreductase	-
FQU83_RS14320	2561130..2561693	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
FQU83_RS14325	2562055..2562765	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T135056 WP_001125475.1 NZ_CP042246:2557624-2558946 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T135056 NZ_CP042246:2557624-2558946 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT135056 WP_001332145.1 NZ_CP042246:2557358-2557624 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT135056 NZ_CP042246:2557358-2557624 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8